Yang Chao, Zhu Zhen, Ouyang Xueyan, Yu Ruihua, Wang Jiawei, Ding Gang, Jiang Feng
Translational Institute for Cancer Pain, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Chongming Branch, Shanghai, China.
School of Life Sciences, Shanghai University, Shanghai, China.
Transl Cancer Res. 2020 Dec;9(12):7519-7530. doi: 10.21037/tcr-20-2115.
The microenvironment of various tumor tissues is acidic. Acid-sensing ion channels (ASICs) are a class of ligand-gated ion channels which are sensitive to extracellular protons and are often highly expressed in tumor tissues. Breast cancer, whose extracellular microenvironment is thought to be acidic, is the most common cancer type among females in the world.
Thirty breast cancer tissues and adjacent normal tissues of patients were collected from 2009 to 2015 at the Xinhua hospital affiliated to Shanghai Jiao Tong University School of Medicine. The expression of acid-sensing ion channel 1a (ASIC1a), a subtype of ASICs family, was detected by immunohistochemistry in breast cancer tissues, and the effect of ASIC1a on the physiological activity of tumor cells was analyzed and experiments.
In this study, it was found that ASIC1a is highly expressed in the tissues of breast cancer patients. experiments revealed that down-regulation of ASIC1a by its antagonist PcTx-1 or ASIC1a siRNA could significantly weaken the migration, proliferation and invasion of tumor cells. studies, down-regulation or inhibition of the ASIC1a could inhibit breast tumor growth.
The high expression of ASIC1a might be related to the enhanced biological activity of breast cancer cells. Whether ASIC1a is a potential therapeutic target for some types of breast cancer deserves further study.
各种肿瘤组织的微环境呈酸性。酸敏感离子通道（ASICs）是一类对细胞外质子敏感的配体门控离子通道，在肿瘤组织中常高表达。乳腺癌是全球女性中最常见的癌症类型，其细胞外微环境被认为是酸性的。
2009年至2015年期间，从上海交通大学医学院附属新华医院收集了30例乳腺癌患者的肿瘤组织及癌旁正常组织。采用免疫组织化学方法检测酸敏感离子通道家族亚型酸敏感离子通道1a（ASIC1a）在乳腺癌组织中的表达情况，并通过实验分析ASIC1a对肿瘤细胞生理活性的影响。
本研究发现，ASIC1a在乳腺癌患者组织中高表达。实验表明，其拮抗剂PcTx-1或ASIC1a siRNA下调ASIC1a可显著削弱肿瘤细胞的迁移、增殖和侵袭能力。研究表明，下调或抑制ASIC1a可抑制乳腺肿瘤生长。
ASIC1a的高表达可能与乳腺癌细胞生物学活性增强有关。ASIC1a是否为某些类型乳腺癌的潜在治疗靶点值得进一步研究。
