Morachis-Valdez Gabriela, Dublán-García Octavio, López-Martínez Leticia Xochitl, Galar-Martínez Marcela, Saucedo-Vence Karinne, Gómez-Oliván Leobardo Manuel
Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón intersección Paseo Tollocan s/n. Col. Residencial Colón, 50120, Toluca, Estado de México, México.
Environ Sci Pollut Res Int. 2015 Jun;22(12):9159-72. doi: 10.1007/s11356-014-4061-7. Epub 2015 Jan 14.
Madín Reservoir (MR) is located on the Río Tlalnepantla in Mexico. Previous studies seeking to identify pollutants at this site evidence that MR water contains a considerable metal load as well as nonsteroidal anti-inflammatory drugs (NSAIDs) at concentrations above those determined suitable for aquatic life. This study aimed to evaluate whether chronic exposure to pollutants in MR alters oxidative stress status and flesh quality in muscle of the common carp Cyprinus carpio. The following biomarkers were evaluated in muscle of carp caught in the general area of discharge from the town of Viejo Madín: hydroperoxide content (HPC), lipid peroxidation (LPX), protein carbonyl content (PCC), and activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). Physicochemical and textural properties of muscle were also evaluated. Results show that the metals Al and Fe were accumulated in muscle of C. carpio at levels of 21.3 and 29.6 μg L(-1), respectively, and the NSAIDs diclofenac, ibuprofen, and naproxen at levels from 0.08 to 0.21 ng L(-1). Fish exposed to discharge from the town of Viejo Madín showed significant increases in HPC (9.77 %), LPX (69.33 %), and PCC (220 %) with respect to control specimens (p < 0.05). Similarly, enzyme activity increased significantly: SOD (80.82 %), CAT (98.03 %), and GPx (49.76 %). In muscle, physicochemical properties evidenced mainly significant reductions compared to control values while textural properties showed significant increases. Thus, water in this reservoir is contaminated with xenobiotics that alter some biological functions in C. carpio, a fish species consumed by the local human population.
马丁水库（MR）位于墨西哥的特拉尔内潘特拉河上。先前旨在确定该地点污染物的研究表明，MR水库的水中含有大量金属以及非甾体抗炎药（NSAIDs），其浓度高于确定的适合水生生物的浓度。本研究旨在评估长期暴露于MR水库中的污染物是否会改变鲤鱼肌肉的氧化应激状态和肉质。对从旧马丁镇排放区域捕获的鲤鱼肌肉中的以下生物标志物进行了评估：过氧化氢含量（HPC）、脂质过氧化（LPX）、蛋白质羰基含量（PCC）以及抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性。还评估了肌肉的物理化学和质地特性。结果表明，金属铝和铁分别以21.3和29.6μg L(-1)的水平在鲤鱼肌肉中积累，非甾体抗炎药双氯芬酸、布洛芬和萘普生的水平为0.08至0.21 ng L(-1)。暴露于旧马丁镇排放物中的鱼，其HPC（9.77%）、LPX（69.33%）和PCC（220%）相对于对照样本显著增加（p < 0.05）。同样，酶活性也显著增加：SOD（80.82%）、CAT（98.03%）和GPx（49.76%）。在肌肉中，物理化学特性主要显示与对照值相比显著降低，而质地特性则显著增加。因此，该水库的水被异生素污染，这些异生素改变了当地人类食用的鲤鱼的一些生物学功能。
