Flood J F, Morley J E
Psychobiology Research Laboratory, Veterans Administration Hospital, Sepulveda, CA 91343.
Peptides. 1989 Sep-Oct;10(5):963-6. doi: 10.1016/0196-9781(89)90176-9.
In mice not deprived of food, centrally administered neuropeptide Y (NPY) increases feeding and improves retention. In this study, we examined the effect of C-terminal NPY fragments on feeding and on memory retention. Mice were trained to avoid footshock in a T-maze. After training NPY, NPY fragments (20-36 and 26-36) or saline were administered intracerebroventricularly. Food consumption was measured during the first hour after training and memory retention was measured one week after training. NPY elicited a 544% increase in feeding compared to the saline control. Neither NPY fragment significantly increased feeding. Both NPY and NPY(20-36) improved retention compared to the saline-treated group. NPY(26-36) did not improve retention. NPY administered to well-trained mice results in amnesia. As a further test of the differential effect of NPY on memory processing and eating, we determined in well-trained mice whether administration of NPY and NPY(20-36) resulted in amnesia. Both NPY and NPY(20-36) resulted in amnesia, but only NPY stimulated feeding. These results are compatible with NPY effects on feeding being mediated through postsynaptic (Y1)NPY receptors and effects on memory retention being mediated through presynaptic (Y2)NPY receptors.
在未禁食的小鼠中，脑室内注射神经肽Y（NPY）可增加进食并改善记忆保持。在本研究中，我们检测了C端NPY片段对进食和记忆保持的影响。将小鼠训练至在T迷宫中避免足部电击。训练后，向小鼠脑室内注射NPY、NPY片段（20 - 36和26 - 36）或生理盐水。在训练后的第一小时测量食物消耗量，并在训练一周后测量记忆保持情况。与生理盐水对照组相比，NPY使进食量增加了544%。两种NPY片段均未显著增加进食量。与生理盐水处理组相比，NPY和NPY（20 - 36）均改善了记忆保持。NPY（26 - 36）未改善记忆保持。给训练良好的小鼠注射NPY会导致失忆。作为对NPY对记忆处理和进食的差异作用的进一步测试，我们在训练良好的小鼠中确定注射NPY和NPY（20 - 36）是否会导致失忆。NPY和NPY（20 - 36）均导致失忆，但只有NPY刺激进食。这些结果与NPY对进食的作用是通过突触后（Y1）NPY受体介导，而对记忆保持的作用是通过突触前（Y2）NPY受体介导相一致。
