Chengappa M M, McLaughlin B G, Kadel W L, Maddux R L, Greer S C
Department of Microbiology, Murray State University, Hopkinsville, KY 42240.
Vet Microbiol. 1989 Dec;21(2):147-54. doi: 10.1016/0378-1135(89)90027-8.
Seventeen Holstein-Friesian calves weighing an average of 139.8 +/- 13.5 (mean +/- standard deviation) kg were used in a study to determine the efficacy of a live vaccine containing of Pasteurella multocida A:3 and Pasteurella haemolytica A:1. Eleven calves received the vaccine by intramuscular injection in the right shoulder, whereas six calves received vaccine diluent and served as non-vaccinated controls. Fourteen days following vaccination (Day 15) all calves were inoculated deep intranasally with 3.6 X 10(7) TCID50 bovine herpes virus-1. On Day 16, calves were stressed by transports, and on Day 17 calves were challenged intratracheally with P. multocida A:3. On Day 22 calves were euthanized and necropsied, and tissues were collected for pathological and microbiological evaluations. Scores were assigned to each calf based on the severity of observed clinical signs. Macroscopic lung lesions were expressed as percentage of tissue involved relative to the total lung tissue of a calf. Plasma fibrinogen concentration, rectal temperature, serum antibody level, microscopic appearance of lung, and microbiologic results were also recorded for analyses. The control calves had significantly higher clinical-sign scores (P less than 0.05) and more severe gross lesions (P less than 0.05) than the vaccinated calves. Although the vaccinated calves had a slight increase of immunoglobulins M and G classes, the differences were not statistically significant (P greater than 0.05, P greater than 0.05). The results of the study indicate that the live Pasteurella vaccine is effective against experimental P. multocida infection in calves.
17头平均体重为139.8±13.5（均值±标准差）千克的荷斯坦 - 弗里生犊牛用于一项研究，以确定一种含有多杀性巴氏杆菌A:3和溶血巴氏杆菌A:1的活疫苗的效力。11头犊牛通过在右肩进行肌肉注射接种疫苗，而6头犊牛接受疫苗稀释剂并作为未接种疫苗的对照。接种疫苗14天后（第15天），所有犊牛经鼻深部接种3.6×10(7) TCID50的牛疱疹病毒1型。在第16天，犊牛因运输而受到应激，在第17天，犊牛经气管内接种多杀性巴氏杆菌A:3。在第22天，犊牛被安乐死并进行剖检，收集组织进行病理学和微生物学评估。根据观察到的临床症状的严重程度为每头犊牛打分。宏观肺损伤以受累组织相对于犊牛总肺组织的百分比表示。还记录血浆纤维蛋白原浓度、直肠温度、血清抗体水平、肺的微观外观和微生物学结果用于分析。对照犊牛的临床症状评分显著更高（P<0.05），肉眼可见的损伤也比接种疫苗的犊牛更严重（P<0.05）。虽然接种疫苗的犊牛的免疫球蛋白M和G类有轻微增加，但差异无统计学意义（P>0.05，P>0.05）。该研究结果表明，这种活的巴氏杆菌疫苗对犊牛实验性多杀性巴氏杆菌感染有效。
