使用五维光声断层扫描技术对整个小鼠大脑中的多个脑血流动力学参数进行无创实时可视化。
Noninvasive real-time visualization of multiple cerebral hemodynamic parameters in whole mouse brains using five-dimensional optoacoustic tomography.
作者信息
Gottschalk Sven, Fehm Thomas Felix, Deán-Ben Xosé Luís, Razansky Daniel
机构信息
Helmholtz Center Munich, Institute for Biological and Medical Imaging (IBMI), Ingolstädter Landstraße 1, Neuherberg, Germany.
1] Helmholtz Center Munich, Institute for Biological and Medical Imaging (IBMI), Ingolstädter Landstraße 1, Neuherberg, Germany [2] Faculty of Medicine, Technical University of Munich, Ismaninger Straße 22, Munich, Germany.
出版信息
J Cereb Blood Flow Metab. 2015 Mar 31;35(4):531-5. doi: 10.1038/jcbfm.2014.249.
Abstract
Current functional neuroimaging methods are not adequate for high-resolution whole-brain visualization of neural activity in real time. Here, we show imaging of fast hemodynamic changes in deep mouse brain using fully noninvasive acquisition of five-dimensional optoacoustic data from animals subjected to oxygenation stress. Multispectral video-rate acquisition of three-dimensional tomographic data enables simultaneous label-free assessment of multiple brain hemodynamic parameters, including blood oxygenation, total hemoglobin, cerebral blood volume, oxygenized and deoxygenized hemoglobin, in real time. The unprecedented results indicate that the proposed methodology may serve as a powerful complementary, and potentially superior, method for functional neuroimaging studies in rodents.
摘要
当前的功能神经成像方法不足以实时对神经活动进行高分辨率全脑可视化。在此，我们展示了利用对处于氧合应激状态的动物进行五维光声数据的完全非侵入式采集，对小鼠深部脑内快速血流动力学变化进行成像。对三维断层数据进行多光谱视频速率采集，能够实时对包括血氧合、总血红蛋白、脑血容量、氧合血红蛋白和脱氧血红蛋白在内的多个脑血流动力学参数进行无标记评估。这些前所未有的结果表明，所提出的方法可能成为啮齿动物功能神经成像研究的一种强大的补充方法，甚至可能是更优越的方法。
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