Talaei-Khozani Tahereh, Borzoei Zahra, Bahmanpour Soghra, Zolghadr Jaleh, Dehbashi Sedigheh, Zareh Hamid Reza
Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Obstetrics and Gynecology, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Reprod Med. 2011 Spring;9(2):125-30.
Recurrent spontaneous abortion impacts almost 1% of couples. The sera from women with unexplained recurrent spontaneous abortion (URSA) have toxic effects on embryos that grow in the uterus. Therefore, the abnormal condition of the uterus may also affect sperm qualities.
The objectives of this study were to search if these sera could induce DNA denaturation in sperm nuclei and also it could reduce sperm motility.
Sera of 20 women with URSA history and sera from 20 women with at least two healthy children were added to the sperms samples from 20 healthy men for 2 hours. The sperm motility was assessed after incubation with sera. The samples were stained with Tdt mediated dUTP nick end labeling (TUNEL) assay for DNA fragmentation. The samples were analyzed with flow cytometry and the percentage of the TUNEL positive sperms were calculated. The data were analyzed by t-test.
The incubation of the sperm samples in sera with URSA lead to a decrease in the percentage of the motile sperm from 55% in control to 41% in the treated group, significantly (p=0.038). The percentage of the sperm with abnormal fragmented DNA increased after incubation with URSA (26.6%) compare to the control (21.2%); however, it was not significant.
It seems that sera from URSA patients could not induce a significant increase in the percentage of the sperms with nuclei contain DNA fragmentation. However, the sera of women with URSA could affect the fertility rate by reduction of the sperm motility.
复发性自然流产影响近1%的夫妇。不明原因复发性自然流产（URSA）女性的血清对子宫内生长的胚胎具有毒性作用。因此，子宫的异常状况也可能影响精子质量。
本研究的目的是探究这些血清是否能诱导精子细胞核中的DNA变性，以及是否会降低精子活力。
将20例有URSA病史女性的血清和20例至少有两个健康孩子的女性的血清加入来自20名健康男性的精子样本中，孵育2小时。与血清孵育后评估精子活力。样本用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法进行DNA片段化染色。用流式细胞仪分析样本并计算TUNEL阳性精子的百分比。数据采用t检验进行分析。
精子样本与URSA血清孵育后，活动精子的百分比从对照组的55%降至处理组的41%，差异有统计学意义（p = 0.038）。与对照组（21.2%）相比，与URSA血清孵育后，DNA片段异常的精子百分比增加（26.6%）；然而，差异无统计学意义。
似乎URSA患者的血清不能显著增加细胞核含有DNA片段的精子百分比。然而，URSA女性的血清可通过降低精子活力来影响生育率。
