Song Jia-Le, Gao Yang
Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, People's Republic of China. ; Department of Food Science and Nutrition, Pusan National University, Busan 609-735, South Korea.
Department of Pharmacy, Northern Jiangsu People's Hospital Affiliated to Yangzhou University (Clinical Medical College of Yangzhou University), Yangzhou, Jiangsu 225001,People's Republic of China.
Iran J Pharm Res. 2014 Fall;13(4):1369-78.
The present study was designed to investigate the protective effect of ethanol extracts from Lindera coreana leaves (LCE) on UVB-induced oxidative stress in HaCaT keratinocytes. The HaCaT cells were pretreated with LCE for 24 h and then exposed to UVB (20 mJ/cm(2)) for 2 h. UVB significantly decreased the cell viability (p<0.05). LCE did not exhibit significantly cytotoxic effects and increased the viability of the HaCaT cells in a concentration-dependent manner. To further investigate the protective effects of LCE on UVB-induced oxidative stress in HaCaT cells, the cellular levels of reactive oxygen species (ROS), lipid peroxidation and endogenous antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px), were analyzed. LCE decreased the intracellular levels of ROS and lipid peroxidation and increased the activity of antioxidant enzymes. These results suggest that LCE exerted cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzymes activities. In addition, LCE also decreased the TNF-α, IL-1β and IL-6 levels in UVB-irradiated HaCaT cells.
本研究旨在探讨香叶树叶片乙醇提取物（LCE）对紫外线B（UVB）诱导的HaCaT角质形成细胞氧化应激的保护作用。将HaCaT细胞用LCE预处理24小时，然后暴露于UVB（20 mJ/cm²）2小时。UVB显著降低了细胞活力（p<0.05）。LCE未表现出明显的细胞毒性作用，并以浓度依赖的方式提高了HaCaT细胞的活力。为了进一步研究LCE对UVB诱导的HaCaT细胞氧化应激的保护作用，分析了细胞内活性氧（ROS）、脂质过氧化水平以及内源性抗氧化酶，包括过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-px）的水平。LCE降低了细胞内ROS和脂质过氧化水平，并提高了抗氧化酶的活性。这些结果表明，LCE通过抑制脂质过氧化、降低ROS水平和刺激抗氧化酶活性，对UVB诱导的HaCaT细胞氧化应激发挥了细胞保护作用。此外，LCE还降低了UVB照射的HaCaT细胞中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）的水平。
