Microbiology Division, CSIR-CLRI, Chennai, Tamil Nadu, India.
J Cell Biochem. 2019 Jun;120(6):10715-10725. doi: 10.1002/jcb.28363. Epub 2019 Jan 28.
The present study explores the UVB protective role of Asperyellone (AY), a secondary metabolite of Aspergillus niger strain AN01. The in vitro UVB protective efficacy of AY was studied using the Human Epidermal keratinocytes cells (HaCaT) cell line. The results suggest the appreciable scavenging of UVB-induced reactive oxygen species in the AY-pretreated cells compared with UVB control. Experimental results on the antioxidant enzymes (Catalase, SOD, LPO, and GPx) profile, histochemical, and molecular analyses support the UVB protective effect of AY in HaCaT cells. Further, the in vivo UVB protective efficacy of AY was studied using animal models and compared with that of commercially available UVB protective agents. Physical, biochemical, and molecular analyses of skin samples emphasized the UVB protective role of AY. Thus, the important beneficial effects of AY have been explored in the present study.
本研究探讨了 Asperyellone（AY）的 UVB 保护作用，AY 是黑曲霉 AN01 菌株的一种次生代谢产物。使用人表皮角质形成细胞（HaCaT）细胞系研究了 AY 的体外 UVB 保护功效。结果表明，与 UVB 对照组相比，AY 预处理细胞中可明显清除 UVB 诱导的活性氧。抗氧化酶（过氧化氢酶、超氧化物歧化酶、LPO 和 GPx）谱、组织化学和分子分析的实验结果支持 AY 在 HaCaT 细胞中的 UVB 保护作用。此外，还使用动物模型研究了 AY 的体内 UVB 保护功效，并与市售的 UVB 保护剂进行了比较。皮肤样本的物理、生化和分子分析强调了 AY 的 UVB 保护作用。因此，本研究探讨了 AY 的重要有益作用。
