Department of Micro- and Nanotechnology, Technical University of Denmark, Oersteds Plads 345 East, DK-2800 Kgs. Lyngby, Denmark; E-Mails:
Biosensors (Basel). 2014 Aug 13;4(3):257-72. doi: 10.3390/bios4030257. eCollection 2014 Sep.
This work describes the electrical investigation of paclitaxel-treated HeLa cells using a custom-made microfluidic biosensor for whole cell analysis in continuous flow. We apply the method of differential electrical impedance spectroscopy to treated HeLa cells in order to elucidate the changes in electrical properties compared with non-treated cells. We found that our microfluidic system was able to distinguish between treated and non-treated cells. Furthermore, we utilize a model for electrical impedance spectroscopy in order to perform a theoretical study to clarify our results. This study focuses on investigating the changes in the electrical properties of the cell membrane caused by the effect of paclitaxel. We observe good agreement between the model and the obtained results. This establishes the proof-of-concept for the application in cell drug therapy.
本工作使用定制的微流控生物传感器对处于连续流动状态下的完整细胞进行分析，描述了紫杉醇处理的 HeLa 细胞的电学研究。我们采用差示阻抗谱法来研究经紫杉醇处理的 HeLa 细胞，以阐明其与未经处理细胞相比在电学性质上的变化。我们发现我们的微流控系统能够区分处理过的细胞和未处理的细胞。此外，我们还利用了一种电阻抗谱模型来进行理论研究，以澄清我们的结果。本研究侧重于研究紫杉醇作用引起的细胞膜电学性质的变化。我们观察到模型与获得的结果之间具有良好的一致性。这为细胞药物治疗的应用提供了概念验证。
