Triplett Lindsay R, Verdier Valérie, Campillo Tony, Van Malderghem Cinzia, Cleenwerck Ilse, Maes Martine, Deblais Loïc, Corral Rene, Koita Ousmane, Cottyn Bart, Leach Jan E
Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO, 80523-1177, USA,
Antonie Van Leeuwenhoek. 2015 Apr;107(4):869-81. doi: 10.1007/s10482-015-0379-5. Epub 2015 Jan 15.
Four bacterial strains, designated M89, M92, M97(T), and M106, were isolated in a previous study from surface-sterilized leaves of rice (Oryza sativa) or murainagrass (Ischaemum rugosum) at three sites in Mali, Africa. Here they were examined by a polyphasic taxonomic approach and analysis of a whole-genome sequence. Phylogenetic analyses based on 16S rRNA sequence and multilocus sequence analysis of seven genes showed that these four strains formed a distinct lineage representing a novel species within the genus Xanthomonas. This was supported by whole-genome average nucleotide identity values calculated from comparisons of strain M97(T) with established Xanthomonas species. The strains can be differentiated from the known Xanthomonas species on the basis of their fatty acid and carbohydrate utilization profiles. Population growth studies on rice confirmed that these bacteria multiply in rice leaves without causing symptoms. Identification of this novel species can be accomplished by using diagnostic primer sets or by gyrB gene sequence analysis. We propose to classify these rice- and grass-associated bacteria as Xanthomonas maliensis sp. nov. with strain M97(T) = CFBP7942(T) = LMG27592(T) as the type strain.
在之前的一项研究中，从非洲马里三个地点的经表面消毒的水稻（Oryza sativa）或鼠尾粟（Ischaemum rugosum）叶片中分离出了四株细菌菌株，分别命名为M89、M92、M97(T)和M106。在此，采用多相分类方法并对全基因组序列进行分析对它们进行了研究。基于16S rRNA序列的系统发育分析和七个基因的多位点序列分析表明，这四株菌株形成了一个独特的谱系，代表了黄单胞菌属内的一个新物种。通过将菌株M97(T)与已确定的黄单胞菌属物种进行比较计算得到的全基因组平均核苷酸同一性值支持了这一点。这些菌株可根据其脂肪酸和碳水化合物利用谱与已知的黄单胞菌属物种区分开来。对水稻的群体生长研究证实，这些细菌在水稻叶片中繁殖而不引起症状。使用诊断引物组或通过gyrB基因序列分析可以完成对这个新物种的鉴定。我们建议将这些与水稻和草相关的细菌归类为新种马利黄单胞菌（Xanthomonas maliensis sp. nov.），以菌株M97(T) = CFBP7942(T) = LMG27592(T)作为模式菌株。
