加拿大艾伯塔省南部用于检测产志贺毒素大肠杆菌的酶免疫测定法和实时聚合酶链反应评估
Evaluation of enzyme immunoassays and real-time PCR for detecting Shiga toxin-producing Escherichia coli in Southern Alberta, Canada.
作者信息
Chui Linda, Patterson-Fortin Laura, Kuo Julie, Li Vincent, Boras Valerie
机构信息
Provincial Laboratory for Public Health, Edmonton, Alberta, Canada University of Alberta, Edmonton, Alberta, Canada
Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
出版信息
J Clin Microbiol. 2015 Mar;53(3):1019-23. doi: 10.1128/JCM.03288-14. Epub 2015 Jan 14.
Abstract
Two immunoassays (Shiga Toxin Chek and Shiga Toxin Quik Chek) and real-time PCR were used to detect Shiga toxin-producing Escherichia coli. For enriched culture, the sensitivity and specificity of the three methods ranged from 80.0% to 98.2% and 98.0% to 100.0%, respectively. STEC isolates were identified in 2.6% of the 784 samples.
摘要
采用两种免疫测定法（志贺毒素检测法和志贺毒素快速检测法）以及实时聚合酶链反应（PCR）来检测产志贺毒素大肠杆菌。对于富集培养物，这三种方法的灵敏度和特异性分别在80.0%至98.2%以及98.0%至100.0%之间。在784份样本中，2.6%的样本鉴定出产志贺毒素大肠杆菌（STEC）菌株。
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