Department of Plant Sciences, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad, 45320, Pakistan.
Environ Sci Pollut Res Int. 2015 Jun;22(12):9275-83. doi: 10.1007/s11356-015-4074-x. Epub 2015 Jan 17.
Microbe-assisted phyto-remediation approach is widely applied and appropriate choice to reduce the environmental risk of heavy metals originated from contaminated soils. The present study was designed to screen out the nested belongings of Eruca sativa plants and Pseudomonas putida (ATCC 39213) at varying cadmium (Cd) levels and their potential to deal with Cd uptake from soils. We carried out pot trial experiment by examining the soil containing E. sativa seedlings either treated with P. putida and/or untreated plants subjected to three different levels (ppm) of Cd (i.e., 150, 250, and 500). In all studied cases, we observed an increase in Cd uptake for E. sativa plants inoculated with P. putida than those of un-inoculated plants. Cd toxicity was assessed by recording different parameters including stunted shoot growth, poor rooting, and Cd residual levels in the plants that were not inoculated with P. putida. Significant difference (p < 0.05) of different growth parameters for inoculated vs non-inoculated plants was observed at all given treatments. However, among the different treatments, E. sativa exhibited increased values for different growth parameters (except proline contents) at lower Cd levels than those of their corresponding higher levels, shoot length (up to 27 %), root length (up to 32 %), whole fresh plant (up to 40 %), dry weight (up to 22 %), and chlorophyll contents (up to 26 %). Despite the hyperaccumulation of Cd in whole plant of E. sativa, P. putida improved the plant growth at varying levels of Cd supply than those of associated non-inoculated plants. Present results indicated that inoculation with P. putida enhanced the Cd uptake potential of E. sativa and favors the healthy growth under Cd stress.
微生物辅助植物修复方法被广泛应用，是降低受污染土壤中重金属环境风险的合适选择。本研究旨在筛选出芸薹属植物和假单胞菌（ATCC 39213）在不同镉（Cd）水平下的嵌套特性及其从土壤中处理 Cd 吸收的潜力。我们通过检查含有芸薹属幼苗的土壤进行盆栽试验，这些土壤要么用假单胞菌处理，要么未处理的植物暴露在三种不同水平（ppm）的 Cd（即 150、250 和 500）下。在所有研究的情况下，我们观察到接种假单胞菌的芸薹属植物对 Cd 的吸收增加，而未接种的植物则减少。通过记录受 Cd 毒性影响的不同参数，包括矮化的茎生长、根系不良和植物中残留的 Cd 水平来评估 Cd 毒性，这些参数在未接种假单胞菌的植物中。在所有处理中，接种和未接种植物的不同生长参数均存在显著差异（p < 0.05）。然而，在不同的处理中，与相应的较高水平相比，芸薹属植物在较低 Cd 水平下表现出不同生长参数（脯氨酸含量除外）的增加值，包括茎长（高达 27%）、根长（高达 32%）、整株鲜重（高达 40%）、干重（高达 22%）和叶绿素含量（高达 26%）。尽管芸薹属植物的整株植物积累了大量的 Cd，但假单胞菌在不同 Cd 供应水平下改善了植物的生长，优于相关的未接种植物。本研究结果表明，接种假单胞菌增强了芸薹属植物对 Cd 的吸收潜力，并有利于在 Cd 胁迫下的健康生长。
