Shafiee Hassan Ali, Motamedi Mohammad Hosein Kalantar, Mina Morteza, Taheri Jamileh Beigom, Azimi Somayyeh, Joharchi Khojasteh, Yadegari Zahra, Rasouli Hamid Reza
Department of Orthodontics, Trauma Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Oral and Maxillofacial Surgery, Trauma Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Anc Sci Life. 2014 Apr-Jun;33(4):203-7. doi: 10.4103/0257-7941.147422.
In Iranian traditional medicine Anbarnesa (derived from smoke from burning female donkey's stool) has been used to treat ulcers and inflammatory conditions like stomatitis and ear infections (otitis). We assess the properties of Anbarnesa as an alternative mouthwash.
In this experimental study, Anbarnesa smoke was analyzed using aGC-mass device. The smoke collected was dissolved at different densities in propylene glycol and incubated in Dulbecco's modified Eagle's medium in direct contact with fibroblast cells. Assessment of cytotoxicity was done at 1, 24 and 72 h. Cell viability was measured by methyl thiazolyl tetrazolium test, and ELISA Reader machine was used to read the results. Data were analyzed using one-way ANOVA test.
The findings of this study showed Anbarnesa was nontoxic in 1/64, 1/128 and 1/256 dilutions. In 1/32 dilution, toxicity was seen after 72 h. In dilutions, 1/8 and 1/16 toxicity were seen in the 1(st) h.
According to the initial results of Anbarnesa may be used as an alternative mouthwash with fewer side-effects for plaque control and prevention of periodontal disease.
在伊朗传统医学中，安巴内萨（源自燃烧母驴粪便产生的烟雾）已被用于治疗溃疡以及口腔炎和耳部感染（中耳炎）等炎症性疾病。我们评估安巴内萨作为一种替代漱口水的特性。
在这项实验研究中，使用气相色谱 - 质谱仪分析安巴内萨烟雾。收集的烟雾以不同浓度溶解在丙二醇中，并在与成纤维细胞直接接触的杜氏改良伊格尔培养基中孵育。在1、24和72小时进行细胞毒性评估。通过甲基噻唑基四氮唑试验测量细胞活力，并使用酶标仪读取结果。数据使用单因素方差分析进行分析。
本研究结果表明，安巴内萨在1/64、1/128和1/256稀释度下无毒。在1/32稀释度下，72小时后出现毒性。在1/8和1/16稀释度下，在第1小时出现毒性。
根据初步结果，安巴内萨可作为一种副作用较少的替代漱口水，用于控制牙菌斑和预防牙周疾病。
