Iravani Siavash, Sajjadi Seyed Ebrahim, Rafieian-Kopaei Mahmoud, Zolfaghari Behzad
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pharmacology, School of Medicine, Medical Plants Research Center Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Adv Biomed Res. 2022 Oct 31;11:91. doi: 10.4103/abr.abr_92_21. eCollection 2022.
Anbarnesa is the female donkey dung typically collected after the labor and in early springtime.
The chemical composition of the smoke collected from Anbarnesa was evaluated by gas chromatography-mass spectrometry (GC/MS), and its antiviral activity was analyzed based on 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay.
As a result, twenty-two constituents representing 97.1% of the Anbarnesa smoke could be identified. Hexadecanoic acid (29.4%), cis-9-octadecenoic acid (17.7%), and octadecanoic acid (10.8%) were the smoke's main constituents, respectively. Antiviral activity was evaluated using MTT assay. The CC value of the compound on Hep2 and Verro cells was 2271.2 μg/mL and 5077.5 μg/mL, respectively. Furthermore, the 50% inhibitory concentration value on adenovirus and herpes simplex type-1 was 802.55 μg/mL and >5077.5, respectively.
it was revealed that Anbarnesa was nontoxic in 1/64, 1/128, and 1/256 dilutions, while the toxicity was detected in 1/32 dilution after 72 h. In addition, in 1/8 and 1/16 dilutions, cell toxicity was identified in the first hour.
安巴内萨是一种通常在分娩后和早春收集的雌性驴粪便。
采用气相色谱-质谱联用仪（GC/MS）对从安巴内萨收集的烟雾的化学成分进行评估，并基于3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）法分析其抗病毒活性。
结果表明，可鉴定出占安巴内萨烟雾97.1%的22种成分。十六烷酸（29.4%）、顺式-9-十八碳烯酸（17.7%）和十八烷酸（10.8%）分别是烟雾的主要成分。使用MTT法评估抗病毒活性。该化合物对Hep2和Verro细胞的CC值分别为2271.2μg/mL和5077.5μg/mL。此外，对腺病毒和单纯疱疹病毒1型的50%抑制浓度值分别为802.55μg/mL和>5077.5。
结果显示，安巴内萨在1/64、1/128和1/256稀释度下无毒，而在72小时后1/32稀释度下检测到毒性。此外，在1/8和1/16稀释度下，在第1小时就发现了细胞毒性。
