Sadeghi Heibatollah, Zarezade Vahid, Sadeghi Hossein, Akbartabar Toori Mehdi, Jafari Barmak Mehrzad, Azizi Arsalan, Ghavamizadeh Mehdi, Mostafazadeh Mostafa
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, IR Iran ; Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IR Iran.
Student Research Committee, Yasuj University of Medical Sciences, Yasuj, IR Iran.
Iran Red Crescent Med J. 2014 Sep 5;16(9):e19259. doi: 10.5812/ircmj.19259. eCollection 2014 Sep.
Stachys pilifera Benth has long been used to treat infectious diseases as well as respiratory and rheumatoid disorders in Iranian folk medicine. Antioxidants, antitumor, and antimicrobial properties of the plant have been reported.
This experimental study was designed to evaluate systemic and topical anti-inflammatory effects of the hydro-alcoholic extract from aerial parts of Stachys pilifera (HESP).
Anti-inflammatory effects of HESP was studied in four well-known animal models of inflammation, including carrageenan- or formalin-induced paw edema in rat (thirteen groups, 6 rats per each group), and 12-O-tetradecanoylphorbol-13-acetate (TPA)- or xylene-induced ear edema in mouse (ten groups, 6 mice per each group). The rats received HESP (50-400 mg/ kg) orally 45 minutes before the subplantar injection of carrageenan or formalin. In TPA or xylene tests, HESP (1, 2.5, and 5 mg/ear) was applied topically simultaneous with these phlogistic agents on the ear mice. Finally, pathological examination of the inflamed tissues (paw and ear) was carried out.
Acute toxicity study of the extract showed that no rats were killed at 5000 mg/kg (LD50 > 5000 mg/kg). The extract (100 and 200 mg/ kg) significantly suppressed carrageenan-induced paw edema 1, 2, 3, and 4 hours after carrageenan challenge in comparison with the control group (P < 0.001). The HESP (100 and 200 mg/kg) also produced a considerable antiedematogenic effect in the formalin test over a period of 24 hours (P < 0.01). Furthermore, topical administration of the HESP (1, 2.5, and 5 mg/ear) inhibited TPA- and xylene-induced ear edema in comparison with the control group (P < 0.001). The pathological analysis of the paws and ears revealed that HESP was capable of reducing tissue destruction, cellular infiltration, and subcutaneous edema induced by the indicated phlogistic agents.
The present data confirmed systemic and topical anti-inflammatory effects of Stachys pilifera which is comparable to indomethacin.
伊朗民间医学长期以来一直使用绵毛水苏来治疗传染病以及呼吸道和类风湿疾病。该植物的抗氧化、抗肿瘤和抗菌特性已有报道。
本实验研究旨在评估绵毛水苏地上部分水醇提取物（HESP）的全身和局部抗炎作用。
在四种著名的炎症动物模型中研究了HESP的抗炎作用，包括角叉菜胶或福尔马林诱导的大鼠足爪水肿（13组，每组6只大鼠），以及12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）或二甲苯诱导的小鼠耳水肿（10组，每组6只小鼠）。大鼠在足底注射角叉菜胶或福尔马林前45分钟口服HESP（50 - 400毫克/千克）。在TPA或二甲苯试验中，将HESP（1、2.5和5毫克/耳）与这些炎症介质同时局部应用于小鼠耳部。最后，对发炎组织（足爪和耳部）进行病理检查。
提取物的急性毒性研究表明，在5000毫克/千克剂量下无大鼠死亡（半数致死量>5000毫克/千克）。与对照组相比，提取物（100和200毫克/千克）在角叉菜胶攻击后1、2、3和4小时显著抑制角叉菜胶诱导的足爪水肿（P<0.001）。HESP（100和200毫克/千克）在福尔马林试验中24小时内也产生了相当大的抗水肿作用（P<0.01）。此外，与对照组相比，局部应用HESP（1、2.5和5毫克/耳）可抑制TPA和二甲苯诱导的耳水肿（P<0.001）。足爪和耳部的病理分析表明，HESP能够减少由所示炎症介质引起的组织破坏、细胞浸润和皮下水肿。
目前的数据证实了绵毛水苏的全身和局部抗炎作用，其作用与吲哚美辛相当。
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