• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Čelak和Benth.水醇提取物的抗氧化和抗癌活性评估

Evaluation of the Antioxidant and Anticancer Activities of Hydroalcoholic Extracts of Čelak and Benth.

作者信息

Barmoudeh Zahra, Ardakani Maryam Tajali, Doustimotlagh Amir Hossein, Bardania Hassan

机构信息

Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.

Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.

出版信息

J Toxicol. 2022 Mar 30;2022:1924265. doi: 10.1155/2022/1924265. eCollection 2022.

DOI:10.1155/2022/1924265
PMID:35401741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8986396/
Abstract

Herein, the effects of hydroalcoholic extracts of Celak (TDC) and Benth (SPB) plants on HepG2 cell line were investigated by using different analyses. Cytotoxicity and apoptosis of extracts were investigated by MTT method, AnnV/PI apoptosis assay, and their antioxidant capacity was evaluated by total thiol and glutathione peroxidase (GPX) assay. The results revealed that the SBP extract was more cytotoxic compared with the TDC extract and increased over time (128.49 g/mL vs 107.11 g/mL IC values for 24 and 72 h, respectively). Although, AnnV/PI apoptosis assay showed apoptosis induction for both extracts, but the caspase-3 activity assay revealed that TDC extract significantly increased caspase-3 activity compared with the control and SPB extract. Increasing the activity of GPX by SPB extract revealed that it has high antioxidant capacity. In conclusion, the TDC and SPB with high antioxidant capacity have high cytotoxicity against HepG2 cancer cells and have high capability as a medicinal plant.

摘要

在此，通过不同分析方法研究了塞拉克（TDC）和本氏（SPB）植物的水醇提取物对HepG2细胞系的影响。采用MTT法、Annexin V/PI凋亡检测法研究提取物的细胞毒性和凋亡情况，并通过总硫醇和谷胱甘肽过氧化物酶（GPX）检测法评估其抗氧化能力。结果显示，与TDC提取物相比，SPB提取物的细胞毒性更强，且随时间增加（24小时和72小时的IC值分别为128.49 μg/mL和107.11 μg/mL）。虽然Annexin V/PI凋亡检测法显示两种提取物均能诱导凋亡，但半胱天冬酶-3活性检测显示，与对照组和SPB提取物相比，TDC提取物显著提高了半胱天冬酶-3的活性。SPB提取物使GPX活性增加，表明其具有较高的抗氧化能力。总之，具有高抗氧化能力的TDC和SPB对HepG2癌细胞具有高细胞毒性，作为药用植物具有很大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/14062bc50305/JT2022-1924265.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/7e96713e01aa/JT2022-1924265.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/9cfa199b8617/JT2022-1924265.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/231922d316cb/JT2022-1924265.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/9cb0c764bf57/JT2022-1924265.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/a49fc38772f0/JT2022-1924265.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/914cc6e3cdd2/JT2022-1924265.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/14062bc50305/JT2022-1924265.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/7e96713e01aa/JT2022-1924265.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/9cfa199b8617/JT2022-1924265.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/231922d316cb/JT2022-1924265.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/9cb0c764bf57/JT2022-1924265.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/a49fc38772f0/JT2022-1924265.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/914cc6e3cdd2/JT2022-1924265.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d132/8986396/14062bc50305/JT2022-1924265.007.jpg

相似文献

1
Evaluation of the Antioxidant and Anticancer Activities of Hydroalcoholic Extracts of Čelak and Benth.Čelak和Benth.水醇提取物的抗氧化和抗癌活性评估
J Toxicol. 2022 Mar 30;2022:1924265. doi: 10.1155/2022/1924265. eCollection 2022.
2
Hepatoprotective and antioxidant activity of hydroalcoholic extract of on acetaminophen-induced liver toxicity in male rats.[提取物名称]水醇提取物对乙酰氨基酚诱导的雄性大鼠肝毒性的保肝和抗氧化活性 。 需注意，原文中“of ”后缺少具体提取物名称，以上译文是补充了提取物名称后的完整表述。
Heliyon. 2019 Dec 24;5(12):e03029. doi: 10.1016/j.heliyon.2019.e03029. eCollection 2019 Dec.
3
Antioxidant and protective effect of Stachys pilifera Benth against nephrotoxicity induced by cisplatin in rats.绵毛水苏对顺铂诱导的大鼠肾毒性的抗氧化及保护作用
J Food Biochem. 2020 May;44(5):e13190. doi: 10.1111/jfbc.13190. Epub 2020 Mar 10.
4
Cytotoxic effect of methanolic extract, alkaloid and terpenoid fractions of against HT-29 cell line.[植物名称]甲醇提取物、生物碱和萜类组分对HT-29细胞系的细胞毒性作用 。 需注意，原文中“against HT-29 cell line”前缺少具体植物名称，这里用“[植物名称]”表示需补充完整信息。
Res Pharm Sci. 2018 Oct;13(5):404-412. doi: 10.4103/1735-5362.236833.
5
Chemical composition, antimicrobial, antioxidant and cytotoxic activity of the essential oil from the leaves of Stachys pilifera Benth.石蚕叶挥发油的化学成分、抗菌、抗氧化和细胞毒性活性研究
FEMS Microbiol Lett. 2021 May 18;368(9). doi: 10.1093/femsle/fnab050.
6
Cytotoxic, antioxidant and antimicrobial effects of nine species of woundwort (Stachys) plants.九种水苏属植物的细胞毒性、抗氧化及抗菌作用
Pharm Biol. 2014 Jan;52(1):62-7. doi: 10.3109/13880209.2013.810650. Epub 2013 Sep 12.
7
Hepatoprotective effect of Stachys pilifera ethanol extract in carbon tetrachloride-induce hepatotoxicity in rats.水苏乙醇提取物对四氯化碳诱导的大鼠肝毒性的肝保护作用。
Pharm Biol. 2017 Dec;55(1):1389-1393. doi: 10.1080/13880209.2017.1302484.
8
Carthamus, Salvia and Stachys species protect neuronal cells against oxidative stress-induced apoptosis.红花、丹参和水苏属植物可保护神经元细胞免受氧化应激诱导的细胞凋亡。
Pharm Biol. 2014 Dec;52(12):1550-7. doi: 10.3109/13880209.2014.908394. Epub 2014 Sep 22.
9
extract inhibits hepatocellular carcinoma via induction of caspase-dependent apoptosis.提取物通过诱导半胱天冬酶依赖性凋亡抑制肝细胞癌。
Onco Targets Ther. 2017 Dec 4;10:5765-5774. doi: 10.2147/OTT.S150092. eCollection 2017.
10
Antioxidant activity, total phenolic and flavonoid contents of some medicinal and aromatic plants used as herbal teas and condiments in Iran.伊朗一些用作草药茶和调味品的药用及芳香植物的抗氧化活性、总酚和黄酮含量。
J Med Food. 2014 Oct;17(10):1151-7. doi: 10.1089/jmf.2013.0057. Epub 2014 Aug 1.

引用本文的文献

1
Regulatory Effects of Extract, Known as Mountain Tea, on miRNA Expression and Its Pharmacokinetic Properties.名为山茶的提取物对miRNA表达及其药代动力学特性的调节作用。
Food Sci Nutr. 2025 May 25;13(6):e70341. doi: 10.1002/fsn3.70341. eCollection 2025 Jun.
2
Identification of Bioactive and Anticancer Properties of Bidens Pilosa in-vitro Evidence.三叶鬼针草的体外生物活性和抗癌特性的鉴定证据。
Asian Pac J Cancer Prev. 2024 Oct 1;25(10):3551-3558. doi: 10.31557/APJCP.2024.25.10.3551.
3
Anthelminthic and antimicrobial effects of hedge woundwort ( L.) growing in Southern Kazakhstan.

本文引用的文献

1
Genus : A Review of Traditional Uses, Phytochemistry and Bioactivity.属：传统用途、植物化学与生物活性综述。
Medicines (Basel). 2020 Sep 29;7(10):63. doi: 10.3390/medicines7100063.
2
The effects of rosmarinic acid on oxidative stress parameters and inflammatory cytokines in lipopolysaccharide-induced peripheral blood mononuclear cells.迷迭香酸对脂多糖诱导的外周血单个核细胞氧化应激参数和炎症细胞因子的影响。
Mol Biol Rep. 2020 May;47(5):3557-3566. doi: 10.1007/s11033-020-05447-x. Epub 2020 Apr 29.
3
Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing.
哈萨克斯坦南部生长的糙苏（L.）的驱虫和抗菌作用。
Front Pharmacol. 2024 May 6;15:1386509. doi: 10.3389/fphar.2024.1386509. eCollection 2024.
4
Anticancer properties and metabolomic profiling of Shorea roxburghii extracts toward gastrointestinal cancer cell lines.对石栗提取物的抗癌特性和代谢组学分析及其对胃肠道癌细胞系的影响。
BMC Complement Med Ther. 2024 Apr 30;24(1):178. doi: 10.1186/s12906-024-04479-1.
5
Velen. Methanolic Extract: In Vitro and In Silico Screening of Its Antimicrobial, Antioxidant, Anti-Quorum Sensing, Antibiofilm, and Anticancer Activities.Velen. 甲醇提取物：其抗菌、抗氧化、抗群体感应、抗生物膜和抗癌活性的体外和计算机模拟筛选
Life (Basel). 2022 Dec 25;13(1):62. doi: 10.3390/life13010062.
新型生物源载银纳米薄膜的简易制备及其在创伤愈合中的内在抗菌和抗氧化活性。
Sci Rep. 2020 Apr 9;10(1):6129. doi: 10.1038/s41598-020-63032-5.
4
Antioxidant and protective effect of Stachys pilifera Benth against nephrotoxicity induced by cisplatin in rats.绵毛水苏对顺铂诱导的大鼠肾毒性的抗氧化及保护作用
J Food Biochem. 2020 May;44(5):e13190. doi: 10.1111/jfbc.13190. Epub 2020 Mar 10.
5
Autophagy: A Potential Therapeutic Target of Polyphenols in Hepatocellular Carcinoma.自噬：多酚在肝细胞癌中的潜在治疗靶点
Cancers (Basel). 2020 Feb 29;12(3):562. doi: 10.3390/cancers12030562.
6
Hepatoprotective and antioxidant activity of watercress extract on acetaminophen-induced hepatotoxicity in rats.西洋菜提取物对乙酰氨基酚诱导的大鼠肝毒性的保肝和抗氧化活性。
Heliyon. 2019 Jul 9;5(7):e02072. doi: 10.1016/j.heliyon.2019.e02072. eCollection 2019 Jul.
7
The Role of Polyphenol (Flavonoids) Compounds in the Treatment of Cancer Cells.多酚（类黄酮）化合物在癌细胞治疗中的作用。
Nutr Cancer. 2020;72(3):386-397. doi: 10.1080/01635581.2019.1637006. Epub 2019 Jul 9.
8
TrxR1, Gsr, and oxidative stress determine hepatocellular carcinoma malignancy.硫氧还蛋白还原酶 1、谷胱甘肽还原酶和氧化应激决定肝癌的恶性程度。
Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11408-11417. doi: 10.1073/pnas.1903244116. Epub 2019 May 16.
9
Hepatocellular Carcinoma.肝细胞癌
N Engl J Med. 2019 Apr 11;380(15):1450-1462. doi: 10.1056/NEJMra1713263.
10
Cytotoxic effect of methanolic extract, alkaloid and terpenoid fractions of against HT-29 cell line.[植物名称]甲醇提取物、生物碱和萜类组分对HT-29细胞系的细胞毒性作用 。 需注意，原文中“against HT-29 cell line”前缺少具体植物名称，这里用“[植物名称]”表示需补充完整信息。
Res Pharm Sci. 2018 Oct;13(5):404-412. doi: 10.4103/1735-5362.236833.