Čelak和Benth.水醇提取物的抗氧化和抗癌活性评估
Evaluation of the Antioxidant and Anticancer Activities of Hydroalcoholic Extracts of Čelak and Benth.
作者信息
Barmoudeh Zahra, Ardakani Maryam Tajali, Doustimotlagh Amir Hossein, Bardania Hassan
机构信息
Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
出版信息
J Toxicol. 2022 Mar 30;2022:1924265. doi: 10.1155/2022/1924265. eCollection 2022.
Herein, the effects of hydroalcoholic extracts of Celak (TDC) and Benth (SPB) plants on HepG2 cell line were investigated by using different analyses. Cytotoxicity and apoptosis of extracts were investigated by MTT method, AnnV/PI apoptosis assay, and their antioxidant capacity was evaluated by total thiol and glutathione peroxidase (GPX) assay. The results revealed that the SBP extract was more cytotoxic compared with the TDC extract and increased over time (128.49 g/mL vs 107.11 g/mL IC values for 24 and 72 h, respectively). Although, AnnV/PI apoptosis assay showed apoptosis induction for both extracts, but the caspase-3 activity assay revealed that TDC extract significantly increased caspase-3 activity compared with the control and SPB extract. Increasing the activity of GPX by SPB extract revealed that it has high antioxidant capacity. In conclusion, the TDC and SPB with high antioxidant capacity have high cytotoxicity against HepG2 cancer cells and have high capability as a medicinal plant.
在此，通过不同分析方法研究了塞拉克（TDC）和本氏（SPB）植物的水醇提取物对HepG2细胞系的影响。采用MTT法、Annexin V/PI凋亡检测法研究提取物的细胞毒性和凋亡情况，并通过总硫醇和谷胱甘肽过氧化物酶（GPX）检测法评估其抗氧化能力。结果显示，与TDC提取物相比，SPB提取物的细胞毒性更强，且随时间增加（24小时和72小时的IC值分别为128.49 μg/mL和107.11 μg/mL）。虽然Annexin V/PI凋亡检测法显示两种提取物均能诱导凋亡，但半胱天冬酶-3活性检测显示，与对照组和SPB提取物相比，TDC提取物显著提高了半胱天冬酶-3的活性。SPB提取物使GPX活性增加，表明其具有较高的抗氧化能力。总之，具有高抗氧化能力的TDC和SPB对HepG2癌细胞具有高细胞毒性，作为药用植物具有很大潜力。
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