感染牛白血病病毒的绵羊血液白细胞及淋巴细胞亚群观察：一项前瞻性研究

Observations on blood leucocytes and lymphocyte subsets in sheep infected with bovine leukaemia virus: a progressive study.

作者信息

Brandon R B, Gatei M H, Naif H M, Daniel R C, Lavin M F

机构信息

Department of Biochemistry, University of Queensland, Brisbane, Australia.

出版信息

Vet Immunol Immunopathol. 1989 Nov 30;23(1-2):15-27. doi: 10.1016/0165-2427(89)90106-2.

DOI:10.1016/0165-2427(89)90106-2

PMID:2559536

Abstract

Haematological parameters and reactivity of lymphocyte antigens to monoclonal antibodies were studied over a 10-month period in sheep experimentally infected with bovine leukaemia virus (BLV). BLV-inoculated animals seroconverted within 1 month and showed a significant lymphocytosis 2-6 weeks after infection. Control animals inoculated with BLV-free lymphocytes showed a stronger and more immediate neutrophil response than those inoculated with BLV-positive lymphocytes. One month after infection, BLV-inoculated sheep showed a relative increase of cells bearing antigens T4, T6, T8 and T19, and 10 months into the trial, MHC II lymphocytes increased, T6 remained elevated, but T4 helper cells were significantly decreased in number. Lymphoma tissue showed the presence of T8 cells, and lymph nodes from seroconverted sheep had areas of concentrated T4 staining cells. These results demonstrate responses in cellular immune mechanisms to infection with BLV.

摘要

在10个月的时间里，对实验感染牛白血病病毒（BLV）的绵羊的血液学参数以及淋巴细胞抗原对单克隆抗体的反应性进行了研究。接种BLV的动物在1个月内血清转化，并在感染后2 - 6周出现明显的淋巴细胞增多。接种无BLV淋巴细胞的对照动物比接种BLV阳性淋巴细胞的动物表现出更强、更迅速的中性粒细胞反应。感染1个月后，接种BLV的绵羊显示携带抗原T4、T6、T8和T19的细胞相对增加，试验进行到10个月时，MHC II淋巴细胞增加，T6仍然升高，但T4辅助细胞数量显著减少。淋巴瘤组织显示存在T8细胞，血清转化绵羊的淋巴结有T4染色细胞集中的区域。这些结果证明了细胞免疫机制对BLV感染的反应。