Aida Y, Miyasaka M, Okada K, Onuma M, Kogure S, Suzuki M, Minoprio P, Levy D, Ikawa Y
Laboratory of Gene Technology and Safety, Tsukuba Life Science Center, Ibaraki, Japan.
Am J Vet Res. 1989 Nov;50(11):1946-51.
To determine the phenotype of target cells for bovine leukemia virus (BLV) infection in sheep, we analyzed blood lymphocytes from BLV-infected clinically healthy and leukemic sheep by use of monoclonal antibodies. In clinically healthy and leukemic sheep that were BLV-infected, the blood concentration of T lymphocytes was within normal values, but the number of B lymphocytes was increased in several cases. In addition, the number of blood lymphocytes expressing the BLV antigen correlated well with that of B lymphocytes. Double immunofluorescence staining demonstrated that lymphocytes expressing BLV antigens bore B-cell but not T-cell surface markers. Moreover, neoplastic cells in the lymph nodes of leukemic sheep were stained immunohistochemically with an anti-B monoclonal antibody but not with any of anti-T monoclonal antibody tested, indicating that tumor cells are of B-lymphocyte origin. Collectively, these results show that BLV antigen-positive cells obtained from BLV-infected sheep that have no clinical signs and BLV-induced lymphosarcoma cells belong to the B-lymphocyte lineage.
为了确定绵羊中牛白血病病毒（BLV）感染的靶细胞表型，我们使用单克隆抗体分析了感染BLV的临床健康绵羊和白血病绵羊的血液淋巴细胞。在感染BLV的临床健康绵羊和白血病绵羊中，T淋巴细胞的血液浓度在正常范围内，但在一些情况下B淋巴细胞数量增加。此外，表达BLV抗原的血液淋巴细胞数量与B淋巴细胞数量密切相关。双重免疫荧光染色表明，表达BLV抗原的淋巴细胞带有B细胞而非T细胞表面标志物。此外，白血病绵羊淋巴结中的肿瘤细胞用抗B单克隆抗体进行免疫组织化学染色，但未用任何测试的抗T单克隆抗体染色，表明肿瘤细胞起源于B淋巴细胞系。总的来说，这些结果表明，从无临床症状的感染BLV的绵羊中获得的BLV抗原阳性细胞和BLV诱导的淋巴肉瘤细胞属于B淋巴细胞系。
