Singh Amarjeet, Kaur Mohanjit, Choudhary Adarsh, Kumar Bimlesh
Department of Pharmaceutical Sciences, Lovely Professional University, Punjab, India.
Pharmacognosy Res. 2015 Jan-Mar;7(1):85-91. doi: 10.4103/0974-8490.147215.
Butea monosperma is a medium sized deciduous tree of family Fabaceae. It is widely used by rural people in India to cure many disorders. It possesses antioxidant and anticancer activity which is a prerequisite for anticlastogenic activity.
To evaluate the effect of Butea monosperma leaf extracts on cyclophosphamide induced clastogenicity and oxidative stress in mice.
The present study assessed the role of aqueous and ethanolic leaf extracts of B. monosperma (AQEBM and ETEBM) on cyclophosphamide (CP) induced oxidative stress and DNA damage in mice using micronucleus assay for anticlastogenic activity and biochemical estimation of malondialdehyde (MDA) and glutathione (GSH) for antioxidant activity. The frequency of the micronucleated erythrocytes and mitotic index was studied in peripheral blood and bone marrow after 24 and 48 h of clastogenic exposure.
CP treatment led to a significant (P < 0.001) increase in the frequency of micronuclei and decrease in the mitotic index (MI) in bone marrow and peripheral blood cells. Moreover, CP also significantly increased the lipid peroxidation as evidenced by an increase in the MDA content and decreased the antioxidant enzyme (GSH) in mice liver. Pretreatment with AQEBM and ETEBM reduced the frequency of micronuclei and increased the MI in the bone marrow and peripheral blood cells and also restored the MDA and GSH levels in mice liver.
The AQEBM and ETEBM do contain compounds capable of inhibiting the CP induced oxidative stress and subsequent DNA damage in both the peripheral blood and bone marrow cells in mice.
紫铆是豆科的一种中型落叶乔木。在印度，农村居民广泛使用它来治疗多种疾病。它具有抗氧化和抗癌活性，而这是抗致突变活性的一个先决条件。
评估紫铆叶提取物对环磷酰胺诱导的小鼠致突变性和氧化应激的影响。
本研究使用微核试验评估紫铆水提物和醇提物（AQEBM和ETEBM）对环磷酰胺（CP）诱导的小鼠氧化应激和DNA损伤的抗致突变活性，并通过生化测定丙二醛（MDA）和谷胱甘肽（GSH）评估其抗氧化活性。在致突变暴露24小时和48小时后，研究外周血和骨髓中微核红细胞的频率和有丝分裂指数。
CP处理导致骨髓和外周血细胞中微核频率显著增加（P < 0.001），有丝分裂指数降低。此外，CP还显著增加了脂质过氧化，表现为小鼠肝脏中MDA含量增加，抗氧化酶（GSH）减少。AQEBM和ETEBM预处理降低了骨髓和外周血细胞中微核的频率，增加了有丝分裂指数，还恢复了小鼠肝脏中MDA和GSH的水平。
AQEBM和ETEBM确实含有能够抑制CP诱导的小鼠外周血和骨髓细胞氧化应激及随后DNA损伤的化合物。
