Aşkin Celik Tülay, Aslantürk Ozlem Sultan
Department of Biology, Faculty of Art and Science, Adnan Menderes University, 09010 Aydin, Turkey.
J Biomed Biotechnol. 2010;2010:189252. doi: 10.1155/2010/189252. Epub 2010 Jun 23.
I. viscosa has been used for years in folk medicine for its anti-inflammatory, antipyretic, antiseptic, and paper antiphlogistic activities. In this study, cytotoxic and genotoxic effects of I. viscosa leaf extracts on the root meristem cells of Allium cepa have been examined. Onion bulbs were exposed to 2.5 mg/ml, 5 mg/ml, and 10 mg/ml concentrations of the extracts for macroscopic and microscopic analysis. Tap water has been used as a negative control and Ethyl methanesulfonate (EMS) (2 * 10(-2) M) has been used as a positive control. The test concentrations have been determined according to doses which are recommended for use in alternative medicine. There has been statistically significant (P < .05) inhibition of root growth depending on concentration by the extracts when compared with the control groups. All the tested extracts have been observed to have cytotoxic effects on cell division in A. cepa. I. viscosa leaf extract induces the total number of chromosomal aberrations and micronuclei (MNC) formations in A. cepa root tip cells significantly when compared with control groups. Also, this paper shows for the first time the induction of cell death, ghost cells, cells with membrane damage, and binucleated cells by extract treatment. These results suggest the cytotoxic and genotoxic effects of the I. viscosa leaf extracts on A. cepa.
多年来，粘毛烟草一直因其抗炎、解热、抗菌和抗纸炎症活性而被用于民间医学。在本研究中，研究了粘毛烟草叶提取物对洋葱根尖分生组织细胞的细胞毒性和遗传毒性作用。将洋葱鳞茎暴露于浓度为2.5毫克/毫升、5毫克/毫升和10毫克/毫升的提取物中，进行宏观和微观分析。自来水用作阴性对照，甲基磺酸乙酯（EMS）（2×10⁻²M）用作阳性对照。测试浓度是根据替代医学中推荐使用的剂量确定的。与对照组相比，提取物对根生长的抑制作用具有统计学意义（P<0.05），且呈浓度依赖性。观察到所有测试提取物对洋葱的细胞分裂均具有细胞毒性作用。与对照组相比，粘毛烟草叶提取物显著诱导洋葱根尖细胞中的染色体畸变总数和微核（MNC）形成。此外，本文首次表明提取物处理可诱导细胞死亡、幽灵细胞、膜损伤细胞和双核细胞。这些结果表明粘毛烟草叶提取物对洋葱具有细胞毒性和遗传毒性作用。
