Müller U, Roberts M P, Engel D A, Doerfler W, Shenk T
Howard Hughes Medical Institute, Department of Biology, Princeton University, New Jersey 08544.
Genes Dev. 1989 Dec;3(12A):1991-2002. doi: 10.1101/gad.3.12a.1991.
Treatment of adenovirus-infected mouse S49 cells with cAMP analogs leads to the transcriptional induction of early viral genes. E1A proteins and cAMP work in synergy to activate several of these genes. We now demonstrate that the transcription factor AP-1 is modestly induced by cAMP in S49 cells and induced to significantly higher levels by cAMP in the presence of E1A proteins. Cytoplasmic levels of c-fos and junB mRNAs are rapidly increased by cAMP, and the induction is substantially stronger in the presence of E1A protein. The AP-1 activity binds efficiently to both AP-1 and activating transcription factor (ATF)/cAMP response element binding protein (CREB)-binding sites present in E1A-inducible promoters and presumably plays a role in the transcriptional activation of adenovirus genes by E1A proteins and cAMP.
用环磷酸腺苷（cAMP）类似物处理腺病毒感染的小鼠S49细胞会导致早期病毒基因的转录诱导。E1A蛋白与cAMP协同作用以激活其中一些基因。我们现在证明，转录因子AP-1在S49细胞中被cAMP适度诱导，而在存在E1A蛋白的情况下，被cAMP诱导到显著更高的水平。cAMP可使c-fos和junB mRNA的细胞质水平迅速升高，并且在存在E1A蛋白的情况下诱导作用明显更强。AP-1活性可有效结合E1A诱导型启动子中存在的AP-1以及激活转录因子（ATF）/环磷酸腺苷反应元件结合蛋白（CREB）结合位点，并且可能在E1A蛋白和cAMP对腺病毒基因的转录激活中发挥作用。
