Tufano M A, Ianniello R, Galdiero M, De Martino L, Galdiero F
Istituto di Microbiologia, I Facoltà di Medicina e Chirurgia, Napoli, Italy.
Microb Pathog. 1989 Nov;7(5):337-46. doi: 10.1016/0882-4010(89)90037-5.
The effect of Salmonella typhimurium porins on human polymorphonuclear leukocytes (PMNs) was studied. Labeled porins were shown to bind to the PMNs, and could be completely displaced by unlabeled porins. The binding caused modifications of membrane integrity and of the physico-chemical characteristics of the PMN surface, e.g. decreased oxidative burst, decreased hydrophobicity and altered cell morphology. The porins acted as both chemotaxins and chemotaxinogens. When PMNs were preincubated with porins their migration in the presence of commonly used chemoattractants (serum activated by zymosan or N-formyl-L-methionyl-L-leucyl-L-phenylalanine) was inhibited.
研究了鼠伤寒沙门氏菌孔蛋白对人多形核白细胞（PMN）的影响。标记的孔蛋白显示可与PMN结合，并且能被未标记的孔蛋白完全取代。这种结合导致膜完整性以及PMN表面物理化学特性的改变，例如氧化爆发减少、疏水性降低和细胞形态改变。孔蛋白兼具趋化因子和趋化因子原的作用。当PMN与孔蛋白预孵育时，它们在常用趋化剂（经酵母聚糖或N-甲酰-L-蛋氨酰-L-亮氨酰-L-苯丙氨酸激活的血清）存在下的迁移受到抑制。
