Ma Rujun, Zhang Yu, Zhang Liang, Han Jun, Rui Rong
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.
Anim Sci J. 2015 Sep;86(9):826-32. doi: 10.1111/asj.12360. Epub 2015 Jan 20.
Sirtuins have been widely reported to be involved in multiple biological processes. However, their function during pig oocyte aging has not been reported yet. Here, we first identify that sirt1 expression is dramatically reduced in pig in vitro-aged oocytes. Furthermore, by confocal scanning and quantitative analysis, we find the increased frequency of spindle defects and chromosome misalignment, disturbed redistribution of cortical granules and mitochondria during oocyte in vitro-aging. Importantly, these aging-associated defective phenotypes can be ameliorated through resveratrol (sirt1 activator) treatment during pig oocyte maturation, providing the evidence for the hypothesis that decreased sirt1 is one of a number of factors contributing to oocyte in vitro-aging. In summary, our data indicate a role for sirt1 in pig oocytes and uncover a striking beneficial effect of sirt1 expression on aged oocytes.
已有广泛报道称，沉默调节蛋白参与多种生物学过程。然而，它们在猪卵母细胞老化过程中的功能尚未见报道。在此，我们首次发现猪体外老化卵母细胞中sirt1的表达显著降低。此外，通过共聚焦扫描和定量分析，我们发现在卵母细胞体外老化过程中，纺锤体缺陷和染色体排列异常的频率增加，皮质颗粒和线粒体的重新分布受到干扰。重要的是，在猪卵母细胞成熟过程中通过白藜芦醇（sirt1激活剂）处理，可以改善这些与老化相关的缺陷表型，为sirt1降低是导致卵母细胞体外老化的众多因素之一这一假说提供了证据。总之，我们的数据表明sirt1在猪卵母细胞中发挥作用，并揭示了sirt1表达对老化卵母细胞具有显著的有益作用。
