Harini Dhandapani, Rajaram Anantanarayanan, Rajaram Rama
Biochemistry Laboratory, Department of Biochemistry, Central Leather Research Institute, Adyar, Chennai, 600 020, India.
J Mater Sci Mater Med. 2015 Jan;26(1):5378. doi: 10.1007/s10856-015-5378-6. Epub 2015 Jan 20.
Monodispersed lanthanum hydroxide nano-rods (LaNRs) were synthesized for prospective biomedical application using a microwave heating and ultrasonic agitation methodology which does not require any toxic stabilizing agent. The average length and diameter of the LaNRs thus obtained were 183.4 ± 3.6 and 9.9 ± 0.2 nm respectively, as analyzed by HRTEM. FTIR spectrum confirmed the presence of OH groups. The thermal transformation of lanthanum hydroxide (La(OH)3) was studied by thermogravimetric analysis. The synthesized LaNRs were found to be stable for a period of 1 month at room temperature. They were biocompatible as evaluated by haemocompatibility assay and viability assay using human peripheral blood mononuclear cells. The pro-angiogenic property of LaNRs was demonstrated by in vivo chick chorioallantoic membrane assay. The LaNRs induced osteoblast differentiation of human adipose derived stem cells with significant calcium (Ca(2+)) deposition indicating potential applications in bone tissue engineering.
采用微波加热和超声搅拌方法合成了单分散氢氧化镧纳米棒（LaNRs），用于潜在的生物医学应用，该方法不需要任何有毒的稳定剂。通过高分辨透射电子显微镜（HRTEM）分析，由此获得的LaNRs的平均长度和直径分别为183.4±3.6纳米和9.9±0.2纳米。傅里叶变换红外光谱（FTIR）证实了羟基的存在。通过热重分析研究了氢氧化镧（La(OH)3）的热转变。发现合成的LaNRs在室温下可稳定保存1个月。通过使用人外周血单核细胞的血液相容性试验和活力试验评估，它们具有生物相容性。通过体内鸡胚绒毛尿囊膜试验证明了LaNRs的促血管生成特性。LaNRs诱导人脂肪来源干细胞的成骨细胞分化，并伴有大量钙（Ca(2+)）沉积，表明其在骨组织工程中具有潜在应用价值。
