Wu Zhiqiang
a Department of Biology , Colorado State University , Fort Collins , CO , USA.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4155-4157. doi: 10.3109/19401736.2014.1003890. Epub 2015 Jan 21.
In this study, we fully assembled eight tomato chloroplast (cp) genomes using next-generation sequencing data. It will provide valuable information for phylogenetic and cp genetic engineering studies of Solanum genus. The total genome size varied from 155,443 to 155,561 bp in length, containing a pair of inverted repeats (IRs) of 25,612-25,639 bp, which were separated by large single copy (LSC) and small single copy (SSC) of 85,857-85,911 bp and 18,362-18,387 bp in size, respectively. The overall GC contents of the chloroplast genome were 37.84-37.88%. The same set of 112 unique genes was annotated from eight cp genomes, including 78 protein coding genes, 30 tRNA genes, and four rRNA genes. Among these, 17 are duplicated in the inverted repeat regions, 15 genes contained one intron, and three genes (rps12, clpP, and ycf3) comprising two introns.
在本研究中，我们利用二代测序数据完整组装了八个番茄叶绿体（cp）基因组。这将为茄属植物的系统发育和叶绿体基因工程研究提供有价值的信息。基因组总长度在155,443至155,561 bp之间，包含一对长度为25,612 - 25,639 bp的反向重复序列（IRs），它们被大小分别为85,857 - 85,911 bp的大单拷贝（LSC）和18,362 - 18,387 bp的小单拷贝（SSC）隔开。叶绿体基因组的总体GC含量为37.84 - 37.88%。从八个叶绿体基因组中注释出了相同的一组112个独特基因，包括78个蛋白质编码基因、30个tRNA基因和4个rRNA基因。其中，17个基因在反向重复区域中重复，15个基因含有一个内含子，3个基因（rps12、clpP和ycf3）含有两个内含子。
