Asaf Sajjad, Waqas Muhammad, Khan Abdul L, Khan Muhammad A, Kang Sang-Mo, Imran Qari M, Shahzad Raheem, Bilal Saqib, Yun Byung-Wook, Lee In-Jung
School of Applied Biosciences, Kyungpook National University Daegu, South Korea.
School of Applied Biosciences, Kyungpook National UniversityDaegu, South Korea; Department of Agriculture, Abdul Wali Khan University MardanMardan, Pakistan.
Front Plant Sci. 2017 Mar 7;8:304. doi: 10.3389/fpls.2017.00304. eCollection 2017.
, a tetraploid wild relative of cultivated rice (family Poaceae), possesses a BBCC genome and contains genes that confer resistance to bacterial blight (BB) and white-backed (WBPH) and brown (BPH) plant hoppers. Based on the importance of this wild species, this study aimed to understand the phylogenetic relationships of with other species through an in-depth analysis of the composition and diversity of the chloroplast (cp) genome. The analysis revealed a cp genome size of 135,094 bp with a typical quadripartite structure and consisting of a pair of inverted repeats separated by small and large single copies, 139 representative genes, and 419 randomly distributed microsatellites. The genomic organization, gene order, GC content and codon usage are similar to those of typical angiosperm cp genomes. Approximately 30 forward, 28 tandem and 20 palindromic repeats were detected in the . cp genome. Comparison of the complete cp genome with another eleven species showed a high degree of sequence similarity and relatively high divergence of intergenic spacers. Phylogenetic analyses were conducted based on the complete genome sequence, 65 shared genes and gene showed same topologies and O. forms a single clade with parental . Thus, the complete . cp genome provides interesting insights and valuable information that can be used to identify related species and reconstruct its phylogeny.
野生稻(禾本科)的四倍体野生近缘种,拥有BBCC基因组,包含赋予对白叶枯病(BB)、白背飞虱(WBPH)和褐飞虱(BPH)抗性的基因。基于该野生种的重要性,本研究旨在通过深入分析叶绿体(cp)基因组的组成和多样性来了解其与其他野生稻物种的系统发育关系。分析揭示了一个大小为135,094 bp的cp基因组,具有典型的四分体结构,由一对被小单拷贝和大单拷贝隔开的反向重复序列、139个代表性基因和419个随机分布的微卫星组成。基因组组织、基因顺序、GC含量和密码子使用与典型被子植物cp基因组相似。在野生稻cp基因组中检测到约30个正向、28个串联和20个回文重复序列。将完整的野生稻cp基因组与另外11个野生稻物种进行比较,显示出高度的序列相似性和基因间隔区的相对高分歧。基于完整基因组序列、65个共享基因进行了系统发育分析,野生稻显示出相同的拓扑结构,并且野生稻与亲本野生稻形成一个单系分支。因此,完整的野生稻cp基因组提供了有趣的见解和有价值的信息,可用于鉴定相关物种并重建其系统发育。