School of Landscape and Architecture, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.
Zhejiang Academy of Forestry, Hangzhou 310023, China.
Molecules. 2018 Apr 8;23(4):846. doi: 10.3390/molecules23040846.
is an important medicinal plant with several pharmacologically active alkaloids and is also used as an ornamental landscape plant. The purpose of this study is to complete and characterize the chloroplast (cp) genome of and compare genomic features to other Myrtales species' cp genomes. The analysis showed that has a total length of 159,219 bp with a typical quadripartite structure containing two identical inverted repeats (IRs) of 25,643 bp isolated by one large single copy (LSC) of 88,571 bp and one small single copy (SSC) of 18,822 bp. The cp genome contains 129 genes with eight ribosomal RNAs, 30 transfer RNAs, and 78 protein coding genes, in which 17 genes are duplicated in two IR regions. The genome organization including gene type and number and guanine-cytosine (GC) content is analyzed among the 12 cp genomes in this study. Approximately 255 simple sequence repeats (SSRs) and 16 forward, two reverses, and two palindromic repeats were identified in the H. myrtifolia cp genome. By comparing the whole cp genome with 11 other Myrtales species, the results showed that the sequence similarity was high between coding regions while sequence divergence was high between intergenic regions. By employing the full cp genomes for phylogenetic analysis, structural and sequence differences were characterized between and 11 Myrtales species illustrating what patterns are common in the evolution of cp genomes within the Myrtales. The first entire cp genome in the genus provides a valuable resource for further studies in these medicinally and ornamentally important taxa.
是一种重要的药用植物，含有几种具有药理活性的生物碱，也被用作观赏景观植物。本研究的目的是完成并描述的叶绿体（cp）基因组，并将基因组特征与其他桃金娘目物种的 cp 基因组进行比较。分析表明，具有 159219bp 的总长度，具有典型的四部分结构，包含两个相同的反向重复（IRs），长度为 25643bp，由一个 88571bp 的大单一拷贝（LSC）和一个 18822bp 的小单一拷贝（SSC）隔开。该 cp 基因组包含 129 个基因，其中 8 个核糖体 RNA、30 个转移 RNA 和 78 个蛋白质编码基因，其中 17 个基因在两个 IR 区域重复。本研究对 12 个 cp 基因组的基因类型、数量和鸟嘌呤-胞嘧啶（GC）含量进行了分析。在 H. myrtifolia cp 基因组中鉴定出约 255 个简单序列重复（SSR）和 16 个正向、2 个反向和 2 个回文重复。通过将整个 cp 基因组与 11 个其他桃金娘目物种进行比较，结果表明编码区域的序列相似性较高，而基因间区的序列差异较大。通过利用完整的 cp 基因组进行系统发育分析，描述了和 11 个桃金娘目物种之间的 cp 基因组的结构和序列差异，说明了 cp 基因组在桃金娘目进化过程中哪些模式是常见的。属内第一个完整的 cp 基因组为这些药用和观赏重要类群的进一步研究提供了有价值的资源。
