通过接种皮肤肿瘤的无细胞提取物增强无毛小鼠紫外线诱导的皮肤癌发生。

Enhancement of u.v.-induced skin carcinogenesis in the hairless mouse by inoculation with cell-free extracts of skin tumours.

作者信息

Reeve V E, Greenoak G E, Canfield P J, Boehm-Wilcox C, Tilbrook P A, Kulski J K, Gallagher C H

机构信息

Department of Veterinary Pathology, University of Sydney, NSW, Australia.

出版信息

Immunol Cell Biol. 1989 Dec;67 ( Pt 6):421-7. doi: 10.1038/icb.1989.59.

DOI:10.1038/icb.1989.59

PMID:2560465

Abstract

The presence of papillomaviral-like DNA has been described in ultraviolet light-induced tumours in the skin of the hairless mouse (14). Here we describe the effects of the inoculation of cell-free extracts of ultraviolet light-induced tumours into the scarified skin of normal hairless mice, prior to exposure of the mice to a cumulative carcinogenic dose of ultraviolet light. Extracts from papillomas or squamous cell carcinomas enhanced the susceptibility of the inoculated mice to ultraviolet light-induced tumorigenesis, if the extracts contained papillomaviral DNA sequences detected by cross-hybridization with Mastomys natalensis papillomaviral DNA. The recipient mice developed a greater tumour incidence, tumour yield, tumour diameter and degree of malignancy.

摘要

在无毛小鼠皮肤的紫外线诱导肿瘤中已发现乳头瘤病毒样DNA（14）。在此，我们描述了在正常无毛小鼠经划痕处理的皮肤中接种紫外线诱导肿瘤的无细胞提取物的效果，然后让小鼠接受累积致癌剂量的紫外线照射。如果来自乳头瘤或鳞状细胞癌的提取物含有通过与南非多乳鼠乳头瘤病毒DNA交叉杂交检测到的乳头瘤病毒DNA序列，那么这些提取物会增强接种小鼠对紫外线诱导肿瘤发生的易感性。受体小鼠出现了更高的肿瘤发生率、肿瘤产量、肿瘤直径和恶性程度。