军团菌属的核糖体RNA基因限制性图谱：一种分子鉴定系统。

rRNA gene restriction patterns of Legionella species: a molecular identification system.

作者信息

Grimont F, Lefèvre M, Ageron E, Grimont P A

机构信息

Unité des Entérobactéries, INSERM Unité 199, Institut Pasteur, Paris.

出版信息

Res Microbiol. 1989 Nov-Dec;140(9):615-26. doi: 10.1016/0923-2508(89)90193-9.

DOI:10.1016/0923-2508(89)90193-9

PMID:2560580

Abstract

A total of 28 species of Legionella could be differentiated by rRNA gene restriction patterns generated after cleavage of total DNA with either EcoRV or HindIII restriction endonucleases, and hybridization of fragments with 32P-labelled Escherichia coli 16 + 23S rRNA. Different species gave different fragment patterns. When several isolates of a species were tested, the patterns obtained were often identical. However, more than one pattern was often observed when more than one serotype was considered. The method should be useful for the identification of all species of Legionella including those exhibiting immunological cross-reactions.

摘要

通过用EcoRV或HindIII限制性内切酶切割总DNA后产生的rRNA基因限制性图谱，以及用32P标记的大肠杆菌16 + 23S rRNA与片段杂交，可以区分总共28种军团菌。不同的物种呈现出不同的片段图谱。当测试一个物种的多个分离株时，获得的图谱通常是相同的。然而，当考虑不止一种血清型时，经常会观察到不止一种图谱。该方法对于鉴定所有军团菌物种应该是有用的，包括那些表现出免疫交叉反应的物种。