Liu Jing, Chen Hu, Ge Wenqi, Wang Yongbo, Sun Yuchun, Wang Yong, Lü Peijun
Center of Digital Dentistry, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing 100081, China.
Academy of Opto-Electronics, Chinese Academy of Sciences, Beijing 100094, China.
J Lasers Med Sci. 2014 Winter;5(1):32-8.
The aim of this study was to evaluate the morphological changes and quantitatively assess the roughness of dentin after the ablation with a Ytterbium-Doped Potassium Yttrium Tungstate (YB: KYW) thin-disk femtosecond pulsed laser of different fluences, scanning speeds and scanning distances.
Twelve extracted human premolars were sectioned into crowns and roots along the cementum-enamel junction, and then the crowns were cut longitudinally into sheets about 1.5 mm thick with a cutting machine. The dentin samples were fixed on a stage at focus plane. The laser beam was irradiated onto the samples through a galvanometric scanning system, so rectangular movement could be achieved. After ablation, the samples were examined with a scanning electron microscope and laser three-dimensional profile measurement microscope for morphology and roughness study.With increasing laser fluence, dentin samples exhibited more melting and resolidification of dentin as well as debris-like structure and occluded parts of dentinal tubules.
When at the scanning speed of 2400mm/s and scanning distance of 24μm, the surface roughness of dentin ablated with femtosecond pulsed laser decreased significantly and varied between values of dentin surface roughness grinded with two kinds of diamond burs with different grits. When at the scanning speed of 1200mm/s and scanning distance of 12μm, the surface roughness decreased slightly, and the surface roughness of dentin ablated with femtosecond pulsed laser was almost equal to that grinded with a low grit diamond bur.
This study showed that increased laser influence may lead to more collateral damage and lower dentin surface roughness, while scanning speed and scanning distance were also negatively correlated with surface roughness. Adequate parameters should be chosen to achieve therapeutic benefits, and different parameters can result in diverse ablation results.
本研究旨在评估用不同能量密度、扫描速度和扫描距离的掺镱钨酸钇钾(YB:KYW)薄盘飞秒脉冲激光消融后牙本质的形态变化,并定量评估其粗糙度。
将12颗拔除的人类前磨牙沿牙骨质-釉质界切割成冠部和根部,然后用切割机将冠部纵向切成约1.5毫米厚的薄片。将牙本质样本固定在焦平面的载物台上。激光束通过振镜扫描系统照射到样本上,从而实现矩形移动。消融后,用扫描电子显微镜和激光三维轮廓测量显微镜检查样本,以进行形态和粗糙度研究。随着激光能量密度的增加,牙本质样本表现出更多的牙本质熔化和再凝固,以及碎屑状结构和牙本质小管的堵塞部分。
当扫描速度为每秒2400毫米且扫描距离为24微米时,用飞秒脉冲激光消融的牙本质表面粗糙度显著降低,且介于用两种不同粒度的金刚石车针研磨的牙本质表面粗糙度值之间。当扫描速度为每秒1200毫米且扫描距离为12微米时,表面粗糙度略有降低,用飞秒脉冲激光消融的牙本质表面粗糙度几乎等于用低粒度金刚石车针研磨的表面粗糙度。
本研究表明,增加激光能量可能导致更多的附带损伤和更低的牙本质表面粗糙度,而扫描速度和扫描距离也与表面粗糙度呈负相关。应选择适当的参数以实现治疗效果,不同的参数可导致不同的消融结果。