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追踪微生物组研究中实验污染的来源。

Tracking down the sources of experimental contamination in microbiome studies.

作者信息

Weiss Sophie, Amir Amnon, Hyde Embriette R, Metcalf Jessica L, Song Se Jin, Knight Rob

出版信息

Genome Biol. 2014 Dec 17;15(12):564. doi: 10.1186/s13059-014-0564-2.

DOI:10.1186/s13059-014-0564-2
PMID:25608874
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4311479/
Abstract

A recent report warns that DNA extraction kits and other laboratory reagents are considerable sources of contamination in microbiome experiments. The issue of contamination is particularly problematic for samples of low biomass.

摘要

最近的一份报告警告说，DNA提取试剂盒和其他实验室试剂是微生物组实验中相当大的污染源。对于低生物量样本来说，污染问题尤其严重。

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本文引用的文献

[1]
Reagent and laboratory contamination can critically impact sequence-based microbiome analyses.

BMC Biol. 2014-11-12

[2]
Evaluating bias of illumina-based bacterial 16S rRNA gene profiles.

Appl Environ Microbiol. 2014-9

[3]
Effect of DNA extraction methods and sampling techniques on the apparent structure of cow and sheep rumen microbial communities.

PLoS One. 2013-9-11

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Meta-analyses of studies of the human microbiota.

Genome Res. 2013-7-16

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A longitudinal study of Streptococcus pneumoniae carriage in a cohort of infants and their mothers on the Thailand-Myanmar border.

PLoS One. 2012-5-31

[6]
Commensal microbiota and myelin autoantigen cooperate to trigger autoimmune demyelination.

Nature. 2011-10-26

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Nat Rev Genet. 2010-9-14

[8]
Sampling and pyrosequencing methods for characterizing bacterial communities in the human gut using 16S sequence tags.

BMC Microbiol. 2010-7-30

[9]
Metabolic syndrome and altered gut microbiota in mice lacking Toll-like receptor 5.

Science. 2010-3-4

[10]
Specific ribosomal DNA sequences from diverse environmental settings correlate with experimental contaminants.

Appl Environ Microbiol. 1998-8

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