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婴幼儿急慢性肠胃炎空肠液及粪便中轮状病毒的鉴定

Rotavirus identification in jejunal juice and stools of acute and chronic forms of infantile gastroenteritis.

作者信息

Candeias J A, Fagundes-Neto U, Rácz M L, Pedra M A, Ferreira V C, Trabulsi L

机构信息

Departamento de Microbiologia, Universidade de São Paulo, Brasil.

出版信息

Braz J Med Biol Res. 1989;22(7):833-9.

PMID:2560940
Abstract
  1. The present study investigates, by means of a combined enzyme immunoassay for rotaviruses and adenoviruses (EIARA), the occurrence of rotaviruses in stools and jejunal juices from 31 children with acute diarrhea and 18 with chronic diarrhea. 2. Stools from 8 acute cases contained rotaviruses (26%). In two of these cases rotaviruses were also detected in the jejunal juice. 3. In the chronic diarrhea group we identified rotaviruses in the stools of one patient and in the jejunal juice of another. 4. Some of the electropherotypes of the rotaviruses identified showed different patterns of RNA migration. 5. Abnormalities of the jejunal mucosa were characterized in 6 acute rotavirus-positive cases. No morphological or functional abnormalities of the intestinal mucosa were observed in the chronic diarrhea rotavirus-positive cases.
摘要
  1. 本研究通过轮状病毒和腺病毒联合酶免疫测定法(EIARA),调查了31例急性腹泻患儿和18例慢性腹泻患儿粪便及空肠液中轮状病毒的感染情况。2. 8例急性腹泻病例的粪便中含有轮状病毒(26%)。其中2例在空肠液中也检测到轮状病毒。3. 在慢性腹泻组中,我们在1例患者的粪便和另1例患者的空肠液中鉴定出轮状病毒。4. 鉴定出的一些轮状病毒的电泳型显示出不同的RNA迁移模式。5. 6例急性轮状病毒阳性病例的空肠黏膜有异常表现。慢性腹泻轮状病毒阳性病例未观察到肠黏膜形态或功能异常。

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