Bäckman Stina, Näslund Jonas, Forsman Mats, Thelaus Johanna
The Swedish Defence Research Agency, FOI Division of CBRN Defence and Security SE- 901 82 Umeå, Sweden.
Sci Rep. 2015 Jan 22;5:7793. doi: 10.1038/srep07793.
Mosquitoes are thought to function as mechanical vectors of Francisella tularensis subspecies holarctica (F. t. holarctica) causing tularemia in humans. We investigated the clinical relevance of transstadially maintained F. t. holarctica in mosquitoes. Aedes egypti larvae exposed to a fully virulent F. t. holarctica strain for 24 hours, were allowed to develop into adults when they were individually homogenized. Approximately 24% of the homogenates tested positive for F. t. DNA in PCR. Mice injected with the mosquito homogenates acquired tularemia within 5 days. This novel finding demonstrates the possibility of transmission of bacteria by adult mosquitoes having acquired the pathogen from their aquatic larval habitats.
蚊子被认为是全北区土拉热弗朗西斯菌（F. t. holarctica）的机械传播媒介，可导致人类兔热病。我们研究了在蚊子体内经跨龄期维持的F. t. holarctica的临床相关性。将埃及伊蚊幼虫暴露于完全有毒力的F. t. holarctica菌株24小时，待其发育成成虫后分别进行匀浆。在PCR检测中，约24%的匀浆F. t. DNA呈阳性。注射蚊子匀浆的小鼠在5天内感染了兔热病。这一新发现表明，成年蚊子从水生幼虫栖息地获得病原体后有传播细菌的可能性。
