自然水体中持久性土拉热弗朗西斯菌全北区亚种的分子检测
Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters.
作者信息
Broman T, Thelaus J, Andersson A-C, Bäckman S, Wikström P, Larsson E, Granberg M, Karlsson L, Bäck E, Eliasson H, Mattsson R, Sjöstedt A, Forsman M
机构信息
Department of CBRN Defence and Security, Swedish Defence Research Agency, 901 82 Umeå, Sweden.
出版信息
Int J Microbiol. 2011;2011. doi: 10.1155/2011/851946. Epub 2010 Sep 8.
Tularemia, caused by the bacterium Francisella tularensis, where F. tularensis subspecies holarctica has long been the cause of endemic disease in parts of northern Sweden. Despite this, our understanding of the natural life-cycle of the organism is still limited. During three years, we collected surface water samples (n = 341) and sediment samples (n = 245) in two areas in Sweden with endemic tularemia. Real-time PCR screening demonstrated the presence of F. tularenis lpnA sequences in 108 (32%) and 48 (20%) of the samples, respectively. The 16S rRNA sequences from those samples all grouped to the species F. tularensis. Analysis of the FtM19InDel region of lpnA-positive samples from selected sampling points confirmed the presence of F. tularensis subspecies holarctica-specific sequences. These sequences were detected in water sampled during both outbreak and nonoutbreak years. Our results indicate that diverse F. tularensis-like organisms, including F. tularensis subsp. holarctica, persist in natural waters and sediments in the investigated areas with endemic tularemia.
兔热病由土拉弗朗西斯菌引起，其中土拉弗朗西斯菌全北区亚种长期以来一直是瑞典北部部分地区地方病的病因。尽管如此，我们对该生物体自然生命周期的了解仍然有限。在三年时间里，我们在瑞典两个有兔热病地方病的地区采集了地表水样本（n = 341）和沉积物样本（n = 245）。实时PCR筛查分别在108份（32%）和48份（20%）样本中检测到土拉弗朗西斯菌lpnA序列。来自这些样本的16S rRNA序列均归为土拉弗朗西斯菌物种。对选定采样点的lpnA阳性样本的FtM19InDel区域进行分析，证实存在土拉弗朗西斯菌全北区亚种特异性序列。在疫情爆发年和非疫情爆发年采集的水样中均检测到了这些序列。我们的结果表明，包括土拉弗朗西斯菌全北区亚种在内的多种类土拉弗朗西斯菌生物体在有兔热病地方病的调查区域的天然水体和沉积物中持续存在。
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