在小鼠模型中使用5-氨基乙酰丙酸聚乳酸-羟基乙酸纳米颗粒介导的光动力疗法治疗皮肤鳞状细胞癌。
Treating cutaneous squamous cell carcinoma using 5-aminolevulinic acid polylactic-co-glycolic acid nanoparticle-mediated photodynamic therapy in a mouse model.
作者信息
Wang Xiaojie, Shi Lei, Tu Qingfeng, Wang Hongwei, Zhang Haiyan, Wang Peiru, Zhang Linglin, Huang Zheng, Zhao Feng, Luan Hansen, Wang Xiuli
机构信息
Shanghai Skin Diseases Clinical College of Anhui Medical University, Shanghai, People's Republic of China ; Shanghai Skin Disease Hospital, Fudan University, Shanghai, People's Republic of China.
Shanghai Skin Disease Hospital, Fudan University, Shanghai, People's Republic of China.
出版信息
Int J Nanomedicine. 2015 Jan 6;10:347-55. doi: 10.2147/IJN.S71245. eCollection 2015.
BACKGROUND
Squamous cell carcinoma (SCC) is a common skin cancer, and its treatment is still difficult. The aim of this study was to evaluate the effectiveness of nanoparticle (NP)-assisted 5-aminolevulinic acid (ALA) delivery for topical photodynamic therapy (PDT) of cutaneous SCC.
MATERIALS AND METHODS
Ultraviolet-induced cutaneous SCCs were established in hairless mice. ALA-loaded polylactic-co-glycolic acid (PLGA) NPs were prepared and characterized. The kinetics of ALA PLGA NP-induced protoporphyrin IX fluorescence in SCCs, therapeutic efficacy of ALA NP-mediated PDT, and immune responses were examined.
RESULTS
PLGA NPs enhanced protoporphyrin IX production in SCC. ALA PLGA NP-mediated topical PDT was more effective than free ALA of the same concentration in treating cutaneous SCC.
CONCLUSION
PLGA NPs provide a promising strategy for delivering ALA in topical PDT of cutaneous SCC.
背景
鳞状细胞癌（SCC）是一种常见的皮肤癌，其治疗仍然具有挑战性。本研究的目的是评估纳米颗粒（NP）辅助递送5-氨基酮戊酸（ALA）用于皮肤鳞状细胞癌局部光动力疗法（PDT）的有效性。
材料与方法
在无毛小鼠中建立紫外线诱导的皮肤鳞状细胞癌。制备并表征了负载ALA的聚乳酸-羟基乙酸共聚物（PLGA）纳米颗粒。检测了ALA PLGA纳米颗粒诱导的鳞状细胞癌中原卟啉IX荧光的动力学、ALA纳米颗粒介导的光动力疗法的治疗效果以及免疫反应。
结果
PLGA纳米颗粒增强了鳞状细胞癌中原卟啉IX的生成。在治疗皮肤鳞状细胞癌方面，ALA PLGA纳米颗粒介导的局部光动力疗法比相同浓度的游离ALA更有效。
结论
PLGA纳米颗粒为皮肤鳞状细胞癌局部光动力疗法中递送ALA提供了一种有前景的策略。
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