Ferreira Bruno G, Carneiro Renê G S, Isaias Rosy M S
Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Pampulha, Caixa Postal 486, CEP 31270-901, Belo Horizonte, MG, Brazil.
Protoplasma. 2015 Sep;252(5):1275-83. doi: 10.1007/s00709-015-0759-8. Epub 2015 Jan 23.
Marcetia taxifolia (A. St.-Hil.) DC. hosts two gall morphotypes, a pistil-shaped gall induced by a Cecidomyiidae (Diptera) and a fusiform stem gall induced by a Lepidoptera. The cytological study of these galls aimed to answer how the difference in nutritive tissues of Diptera and Lepidoptera galls could be explained on cytological basis. The nutritive tissues of lepidopteran galls have a fast-dividing cell zone, the storage nutritive tissue, which replaces the cells of the typical nutritive tissue, where the larvae feed. The differentiation of multivesicular bodies in the plasma membrane occurred exclusively in these fast-dividing cells of the lepidopteran galls, evidencing the meristematic condition of such tissue. The accumulation of reactive oxygen species (ROS) analyzed in situ in the nutritive cells is not sufficient to induce programmed cell death (PCD), as the cells of M. taxifolia have plastoglobules and accumulate polyphenols and terpenoids, which are diagnostic defenses against oxidative stress. The two taxa of galling insects have different nutritional requirements, thus inducing specific cytoplasm-enriched cells on their nutritive tissues.
小叶马尔切蒂木（Marcetia taxifolia (A. St.-Hil.) DC.）上有两种瘿形态类型，一种是由瘿蚊科（双翅目）诱导形成的雌蕊形瘿，另一种是由鳞翅目诱导形成的纺锤形茎瘿。对这些瘿的细胞学研究旨在回答如何从细胞学角度解释双翅目和鳞翅目瘿营养组织的差异。鳞翅目瘿的营养组织有一个快速分裂的细胞区，即储存营养组织，它取代了幼虫取食的典型营养组织的细胞。质膜中多泡体的分化仅发生在鳞翅目瘿的这些快速分裂细胞中，证明了这种组织的分生状态。在营养细胞中原位分析的活性氧（ROS）积累不足以诱导程序性细胞死亡（PCD），因为小叶马尔切蒂木的细胞有质体小球并积累多酚和萜类化合物，这些是对抗氧化应激的诊断性防御物质。两种瘿蜂类昆虫有不同的营养需求，因此在其营养组织上诱导出特定的富含细胞质的细胞。
