Baio Gabriella, Rescinito Giuseppe, Rosa Francesca, Pace Daniele, Boccardo Simona, Basso Luca, Salvi Sandra, Calabrese Massimo, Truini Mauro, Neumaier Carlo Emanuele
Diagnostic Imaging and Senology Unit, IRCCS Azienda Ospedaliera Universitaria (AOU) San Martino-IST-National Cancer Institute, 16132, Genoa, Italy,
Mol Imaging Biol. 2015 Aug;17(4):548-56. doi: 10.1007/s11307-015-0823-y.
The calcium-sensing receptor (CaSR) is overexpressed in many pathological states including breast cancer. Since choline kinase may be activated in breast cancer cells by CaSR resulting in increased phosphocholine production, we sought to correlate the total choline peak in breast lesions as measured by in vivo proton magnetic resonance spectroscopy ((1)H-MRS) with the CaSR expression levels in surgical specimens.
Thirty-six patients with breast lesions were MR scanned at 3T scanner. Tumour morphology and DCE-MR kinetics were evaluated. (1)H-MRS was applied for Cho detection and compared with the CaSR immunohistochemistry analysis (score 0-5) on surgical breast specimens.
Thirty-four lesions demonstrated a DCE malignant kinetics curve (types 2 and 3), while two lesions showed a benign (type 1). Twenty of the 23 breast cancer lesions (87%) with a consistent Cho peak expressed a CaSR score of 3-5, and ten of the 11 breast lesions negative for Cho (91%) had a CaSR score of 1-2. The two benign lesions showed a non-uniform/weak intense expression of the CaSR (score 3) with a consistent Cho peak.
The presence or absence of choline peak evaluated by (1)H-MRS, well correlated with the expression of CaSR in patients with breast lesions (p < 0.01), supports the hypothesis that CaSR may play an important role in the production of choline in breast cancer.
钙敏感受体（CaSR）在包括乳腺癌在内的多种病理状态下过度表达。由于胆碱激酶可能在乳腺癌细胞中被CaSR激活，导致磷酸胆碱生成增加，我们试图将通过体内质子磁共振波谱（（1）H-MRS）测量的乳腺病变中总胆碱峰与手术标本中CaSR的表达水平相关联。
36例乳腺病变患者在3T扫描仪上进行磁共振扫描。评估肿瘤形态和动态对比增强磁共振成像（DCE-MR）动力学。应用（1）H-MRS检测胆碱，并与乳腺手术标本的CaSR免疫组织化学分析（评分0-5）进行比较。
34个病变显示DCE恶性动力学曲线（2型和3型），而2个病变显示良性（1型）。23个有一致胆碱峰的乳腺癌病变中有20个（87%）CaSR评分为3-5，11个胆碱阴性的乳腺病变中有10个（91%）CaSR评分为1-2。两个良性病变显示CaSR表达不均匀/强度较弱（评分3），有一致的胆碱峰。
通过（1）H-MRS评估的胆碱峰的有无与乳腺病变患者CaSR的表达密切相关（p < 0.01），支持CaSR可能在乳腺癌胆碱生成中起重要作用的假说。
