锰增强磁共振成像（MEMRI）在乳腺癌和前列腺癌中的应用：探索钙受体潜在作用的初步研究结果。
Manganese-enhanced MRI (MEMRI) in breast and prostate cancers: Preliminary results exploring the potential role of calcium receptors.
机构信息
NNUH-Norfolk and Norwich University Hospital and Norwich Medical School, University of East Anglia, Norwich, United Kingdom.
Diagnostic Imaging and Senology Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
出版信息
PLoS One. 2020 Sep 15;15(9):e0224414. doi: 10.1371/journal.pone.0224414. eCollection 2020.
PROCEDURES
To preliminary assess the relationship between Manganese Enhanced Magnetic Resonance Imaging (MEMRI) and the expression of calcium receptors in human prostate and breast cancer animal models.
METHODS
NOD/SCID mice were inoculated with MDA-MB-231 breast cancer cells and prostate PC3 cancer cells to develop orthotopic or pseudometastatic cancer animal models. Mice were studied on a clinical 3T scanner by using a prototype birdcage coil before and after intravenous injection of MnCl2. Assessment of receptor's status was carried out after the MR images acquisition by immunohistochemistry on excised tumours.
RESULTS
Manganese contrast enhancement in breast or prostate cancer animal models well correlated with CaSR expression (p<0.01), whereas TRPV6 expression levels appeared not relevant to the Mn uptake.
CONCLUSION
Our preliminary results suggest that MEMRI appears an efficient tool to characterize human breast and prostate cancer animal models in the presence of different expression level of calcium receptors.
方法
将 MDA-MB-231 乳腺癌细胞和前列腺 PC3 癌细胞接种到 NOD/SCID 小鼠体内，以建立原位或假性转移的癌症动物模型。在静脉注射 MnCl2 前后，使用原型鸟笼线圈，在临床 3T 扫描仪上对小鼠进行研究。通过对切除肿瘤的免疫组织化学检测，在获得 MR 图像后对受体状态进行评估。
结果
乳腺癌或前列腺癌动物模型中的锰对比增强与 CaSR 表达密切相关（p<0.01），而 TRPV6 表达水平似乎与锰摄取无关。
结论
我们的初步结果表明，MEMRI 似乎是一种有效的工具，可以在不同钙受体表达水平下对人乳腺癌和前列腺癌动物模型进行特征描述。
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