Candida dubliniensis and Candida albicans differentiation by colony morphotype in Sabouraud-triphenyltetrazolium agar.
作者信息
Gamarra Soledad, Mancilla Estefanía, Dudiuk Catiana, Garcia-Effron Guillermo
机构信息
Laboratorio de Micología y Diagnóstico Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CONICET, Santa Fe, Argentina; Catedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
Laboratorio de Micología y Diagnóstico Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CONICET, Santa Fe, Argentina; Catedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
出版信息
Rev Iberoam Micol. 2015 Apr-Jun;32(2):126-8. doi: 10.1016/j.riam.2014.02.002. Epub 2014 Aug 29.
BACKGROUND
Candida dubliniensis is a germ tube and chlamydoconidia producing Candida species that may be misidentified as Candida albicans. Molecular-based methods are the most reliable techniques for C. albicans and C. dubliniensis differentiation. However, accurate, quick and inexpensive phenotypic tests are needed to be used in low-complexity mycology laboratories.
AIMS
To evaluate colony morphotypes on Sabouraud-triphenyltetrazolium agar as a tool for C. dubliniensis and C. albicans differentiation.
METHODS
The morphology of 126 C. albicans and C. dubliniensis strains was evaluated and compared with their identification by molecular methods.
RESULTS
The method showed 100% sensitivity and specificity when color and the presence or absence of large white mycelial halo was evaluated.
CONCLUSIONS
Colony morphotype on Sabouraud-triphenyltetrazolium agar should be considered as a new tool to differentiate C. dubliniensis and C. albicans.
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