Pinjon E, Sullivan D, Salkin I, Shanley D, Coleman D
Department of Oral Medicine and Pathology, School of Dental Science and Dublin Dental Hospital, Trinity College, University of Dublin, Republic of Ireland.
J Clin Microbiol. 1998 Jul;36(7):2093-5. doi: 10.1128/JCM.36.7.2093-2095.1998.
Candida dubliniensis is a recently described pathogenic species which shares many phenotypic features with Candida albicans, including the ability to form germ tubes and chlamydospores. These similarities have caused significant problems in the identification of C. dubliniensis by the average clinical mycology laboratory. To facilitate the differentiation of these species, we investigated the growth of 120 isolates of C. dubliniensis and 98 C. albicans isolates at 42 and 45 degrees C on Emmons' modified Sabouraud glucose agar (SGA) and 10 isolates of each species in yeast-peptone-dextrose broth. None of the C. dubliniensis isolates grew on the agar or in the broth medium at 45 degrees C, while 11 isolates were capable of growing on SGA at 42 degrees C. In contrast, all of the C. albicans isolates but one grew at 45 degrees C on or in either medium. These reproducible results clearly demonstrate that the incubation of isolates suspected to be C. dubliniensis or C. albicans at 45 degrees C provides a simple, reliable, and inexpensive method for the differentiation of the two species.
都柏林念珠菌是一种最近才被描述的致病菌种，它与白色念珠菌有许多表型特征相同，包括形成芽管和厚壁孢子的能力。这些相似性给普通临床真菌学实验室鉴定都柏林念珠菌带来了重大问题。为便于区分这两个菌种，我们研究了120株都柏林念珠菌和98株白色念珠菌在42℃和45℃条件下，在艾氏改良沙氏葡萄糖琼脂（SGA）上的生长情况，以及每种菌种各10株在酵母蛋白胨葡萄糖肉汤中的生长情况。所有都柏林念珠菌分离株在45℃时均不能在琼脂或肉汤培养基上生长，而有11株能够在42℃的SGA上生长。相比之下，除一株外，所有白色念珠菌分离株在45℃时均能在两种培养基中的一种或两种上生长。这些可重复的结果清楚地表明，将疑似都柏林念珠菌或白色念珠菌的分离株在45℃下培养，为区分这两个菌种提供了一种简单、可靠且廉价的方法。
