Esposito Susanna, Terranova Leonardo, Ruggiero Luca, Ascolese Beatrice, Montinaro Valentina, Rios Walter Peves, Galeone Carlotta, Principi Nicola
Pediatric Highly Intensive Care Unit, Department of Pathophysiology and Transplantation, Università degli Studi di Milano, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy.
J Med Microbiol. 2015 Apr;64(Pt 4):427-431. doi: 10.1099/jmm.0.000029. Epub 2015 Jan 22.
Streptococcus pneumoniae and Staphylococcus aureus are common commensals of the upper respiratory tract in children and adolescents. Understanding the relationship between these two pathogens, including their potential for mutual interference, is needed to evaluate the epidemiology of the diseases they cause, the factors that condition acquisition and carriage, and the impact of related preventative measures. We obtained oropharyngeal and nasal swabs from 497 healthy subjects aged 6-17 years. S. pneumoniae detection and serotyping were performed using a real-time PCR and S. aureus detection was performed using the RIDAGENE MRSA system. We found that 136 (27.3%) of the children were carriers of both species, 121 (24.3%) of the children carried S. pneumoniae alone and 128 (25.7%) of the children carried S. aureus alone. S. aureus carriage was similar between children who carried S. pneumoniae (136/257, 52.9 %, 95% confidence interval [CI]: 46.8-58.9%) vs those who did not (128/240, 53.3%, 95% CI: 47.0 -59.5%) and was independent of age and vaccination with 7-valent pneumococcal conjugate vaccine (PCV7). Vaccination with PCV7 did not affect S. aureus carriage [S. pneumoniae: 84/143 (58.7%, 95% CI: 50.5 -66.5%) vaccinated children vs 171/351 (48.7%, 95% CI: 43.5 -53.9%) unvaccinated children; S. aureus: 67/143 (46.9%, 95% CI: 38.9-55.0 %) vaccinated children vs 195/351 (55.6%, 95% CI: 50.3 -60.7%) unvaccinated children]. Pneumococcal serotype also did not appear to affect S. aureus carriage. These findings suggested that the carriage of S. pneumoniae did not affect that of S. aureus in older children and adolescents, regardless of age, PCV7 vaccination and pneumococcal serotype.
肺炎链球菌和金黄色葡萄球菌是儿童和青少年上呼吸道常见的共生菌。为了评估这两种病原体所引发疾病的流行病学、影响其感染和携带的因素以及相关预防措施的效果，有必要了解它们之间的关系，包括相互干扰的可能性。我们从497名6至17岁的健康受试者中采集了口咽和鼻拭子。采用实时聚合酶链反应（PCR）进行肺炎链球菌检测和血清分型，使用RIDAGENE MRSA系统进行金黄色葡萄球菌检测。我们发现，136名（27.3%）儿童同时携带这两种菌，121名（24.3%）儿童仅携带肺炎链球菌，128名（25.7%）儿童仅携带金黄色葡萄球菌。携带肺炎链球菌的儿童中金黄色葡萄球菌的携带率（136/257，52.9%，95%置信区间[CI]：46.8 - 58.9%）与未携带肺炎链球菌的儿童相似（128/240，53.3%，95% CI：47.0 - 59.5%），且与年龄和7价肺炎球菌结合疫苗（PCV7）接种无关。接种PCV7不影响金黄色葡萄球菌的携带情况[肺炎链球菌：接种疫苗的儿童中84/143（58.7%，95% CI：50.5 - 66.5%），未接种疫苗的儿童中171/351（48.7%，95% CI：43.5 - 53.9%）；金黄色葡萄球菌：接种疫苗的儿童中67/143（46.9%，95% CI：38.9 - 55.0%），未接种疫苗的儿童中195/351（55.6%，95% CI：50.3 - 60.7%）]。肺炎球菌血清型似乎也不影响金黄色葡萄球菌的携带情况。这些研究结果表明，在大龄儿童和青少年中，肺炎链球菌的携带情况不影响金黄色葡萄球菌的携带情况，无论年龄、PCV7接种情况及肺炎球菌血清型如何。
