Alzweiri Muhammed, Alrawashdeh Ibrahim M, Bardaweel Sanaa K
Department of Pharmaceutical Sciences, Faculty of Pharmacy, The University of Jordan, Amman 11942, Jordan.
Department of Biological Sciences, Faculty of Sciences, Al-Hussein Bin Talal University, Ma'an 20, Jordan.
Int J Anal Chem. 2014;2014:627038. doi: 10.1155/2014/627038. Epub 2014 Dec 29.
Artemisia judaica L. is a medicinal plant that is traditionally used to relieve abdominal pains through its carminative activity. In this study, spectroscopic analysis was employed to investigate the carminative activity associated with A. judaica. Using infrared spectroscopy, the carminative activity was evaluated based on the first derivative of IR-characteristic stretching signal of CO2. Our results indicate that A. judaica oil effectively reduced the response of CO2 signal equivalent to thymol standard. Additionally, (1)H-NMR spectroscopy was utilized to assess surface activity of A. judaica crude oil through the reduction of interfacial tension in a D2O/CDCl3 system. Apparently, 10 mg of the oil was able to solubilize water in a chloroform layer up to 4.3% (w/w). In order to correlate the observed surface activity of the oil to its actual composition, GC-MS and GC-FID structural analysis were undertaken. The results revealed that the oil composition consists of oxygenated terpenes which might be responsible for the carminative effect. Furthermore, owing to its sensitivity, our model provides a fundamental basis for the pharmacological assessment of trace amounts of oils with high precision and accuracy.
犹太蒿是一种药用植物，传统上用于通过其驱风作用缓解腹痛。在本研究中，采用光谱分析来研究与犹太蒿相关的驱风活性。利用红外光谱，基于二氧化碳的红外特征伸缩信号的一阶导数来评估驱风活性。我们的结果表明，犹太蒿油有效地降低了与百里酚标准相当的二氧化碳信号响应。此外，利用核磁共振氢谱通过降低D2O/CDCl3体系中的界面张力来评估犹太蒿原油的表面活性。显然，10毫克该油能够使氯仿层中的水溶解至4.3%（w/w）。为了将观察到的油的表面活性与其实际成分相关联，进行了气相色谱 - 质谱联用和气相色谱 - 氢火焰离子化检测器结构分析。结果表明，油的成分由含氧萜类化合物组成，这可能是其驱风作用的原因。此外，由于其灵敏度，我们的模型为高精度和准确地药理评估痕量油提供了基础。
