Lucena Eudes Euler de Souza, Guzen Fausto Pierdoná, Cavalcanti José Rodolfo Lopes de Paiva, Marinho Maria Jocileide de Medeiros, Pereira Wogelsanger Oliveira, Barboza Carlos Augusto Galvão, Costa Miriam Stela Mariz de Oliveira, do Nascimento Júnior Expedito Silva, Cavalcante Jeferson Sousa
Laboratory of Experimental Neurology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, Brazil.
Laboratory of Molecular Biology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, Brazil.
ScientificWorldJournal. 2014;2014:457380. doi: 10.1155/2014/457380. Epub 2014 Dec 29.
A number of evidences show the influence of the growth of injured nerve fibers in peripheral nervous system as well as potential implant stem cells (SCs). The SCs implementation in the clinical field is promising and the understanding of proliferation and differentiation is essential. This study aimed to evaluate the plasticity of mesenchymal SCs from bone marrow of mice in the presence of culture medium conditioned with facial nerve explants and fibroblast growth factor-2 (FGF-2). The growth and morphology were assessed for over 72 hours. Quantitative phenotypic analysis was taken from the immunocytochemistry for glial fibrillary acidic protein (GFAP), protein OX-42 (OX-42), protein associated with microtubule MAP-2 (MAP-2), protein β-tubulin III (β-tubulin III), neuronal nuclear protein (NeuN), and neurofilament 200 (NF-200). Cells cultured with conditioned medium alone or combined with FGF-2 showed morphological features apparently similar at certain times to neurons and glia and a significant proliferative activity in groups 2 and 4. Cells cultivated only with conditioned medium acquired a glial phenotype. Cells cultured with FGF-2 and conditioned medium expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. This study improves our understanding of the plasticity of mesenchymal cells and allows the search for better techniques with SCs.
大量证据表明外周神经系统中受损神经纤维的生长以及潜在的植入干细胞（SCs）的影响。SCs在临床领域的应用前景广阔，了解其增殖和分化至关重要。本研究旨在评估在面神经外植体和碱性成纤维细胞生长因子-2（FGF-2）条件培养基存在的情况下，小鼠骨髓间充质SCs的可塑性。对其生长和形态进行了超过72小时的评估。通过免疫细胞化学对胶质纤维酸性蛋白（GFAP）、蛋白OX-42（OX-42）、微管相关蛋白2（MAP-2）、蛋白β-微管蛋白III（β-微管蛋白III）、神经元核蛋白（NeuN）和神经丝200（NF-200）进行定量表型分析。单独用条件培养基或与FGF-2联合培养的细胞在某些时候表现出与神经元和神经胶质明显相似的形态特征，并且在第2组和第4组中有显著的增殖活性。仅用条件培养基培养的细胞获得了神经胶质表型。用FGF-2和条件培养基培养的细胞表达GFAP、OX-42、MAP-2、β-微管蛋白III、NeuN和NF-200。本研究提高了我们对间充质细胞可塑性的理解，并有助于寻找更好的SCs技术。
