CIMAR/CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 177, 4050-123 Porto, Portugal.
CIMAR/CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 177, 4050-123 Porto, Portugal; Escola Superior de Tecnologia de Saúde do Porto, Instituto Politécnico do Porto, Porto, Portugal.
Ecotoxicol Environ Saf. 2015 Apr;114:67-74. doi: 10.1016/j.ecoenv.2015.01.008. Epub 2015 Jan 21.
Early life-stage bioassays have been used as an alternative to short-term adult toxicity tests since they are cost-effective. A single couple can produce hundreds or thousands of embryos and hence can be used as a simple high-throughput approach in toxicity studies. In the present study, zebrafish and sea urchin embryo bioassays were used to test the toxicity of four pharmaceuticals belonging to different therapeutic classes: diclofenac, propranolol, simvastatin and sertraline. Simvastatin was the most toxic tested compound for zebrafish embryo, followed by diclofenac. Sertraline was the most toxic drug to sea urchin embryos, inducing development abnormalities at the ng/L range. Overall, our results highlight the potential of sea urchin embryo bioassay as a promising and sensitive approach for the high-throughput methods to test the toxicity of new chemicals, including pharmaceuticals, and identify several drugs that should go through more detailed toxicity assays.
早期生命阶段生物测定法已被用作短期成人毒性测试的替代方法，因为它们具有成本效益。一对夫妇可以产生数百个或数千个胚胎，因此可以作为毒性研究中的一种简单高通量方法。在本研究中，使用斑马鱼和海胆胚胎生物测定法来测试属于不同治疗类别的四种药物的毒性：双氯芬酸、普萘洛尔、辛伐他汀和舍曲林。辛伐他汀是对斑马鱼胚胎最具毒性的测试化合物，其次是双氯芬酸。舍曲林是对海胆胚胎最毒的药物，在 ng/L 范围内诱导发育异常。总体而言，我们的结果强调了海胆胚胎生物测定法作为一种有前途和敏感的高通量方法的潜力，可用于测试新化学物质（包括药物）的毒性，并确定几种应通过更详细毒性测定的药物。
