Deshmukh Atul S, Murgia Marta, Nagaraj Nagarjuna, Treebak Jonas T, Cox Jürgen, Mann Matthias
From the ‡Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany;
From the ‡Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany; §Department of Biomedical Sciences, University of Padova, Viale G. Colombo 3, 35121 Padua, Italy;
Mol Cell Proteomics. 2015 Apr;14(4):841-53. doi: 10.1074/mcp.M114.044222. Epub 2015 Jan 22.
Skeletal muscle constitutes 40% of individual body mass and plays vital roles in locomotion and whole-body metabolism. Proteomics of skeletal muscle is challenging because of highly abundant contractile proteins that interfere with detection of regulatory proteins. Using a state-of-the art MS workflow and a strategy to map identifications from the C2C12 cell line model to tissues, we identified a total of 10,218 proteins, including skeletal muscle specific transcription factors like myod1 and myogenin and circadian clock proteins. We obtain absolute abundances for proteins expressed in a muscle cell line and skeletal muscle, which should serve as a valuable resource. Quantitation of protein isoforms of glucose uptake signaling pathways and in glucose and lipid metabolic pathways provides a detailed metabolic map of the cell line compared with tissue. This revealed unexpectedly complex regulation of AMP-activated protein kinase and insulin signaling in muscle tissue at the level of enzyme isoforms.
骨骼肌占个体体重的40%，在运动和全身代谢中发挥着至关重要的作用。由于高丰度的收缩蛋白会干扰调节蛋白的检测，骨骼肌蛋白质组学具有挑战性。我们使用了先进的质谱工作流程以及将C2C12细胞系模型中的鉴定结果映射到组织的策略，共鉴定出10218种蛋白质，包括骨骼肌特异性转录因子如肌分化抗原1（Myod1）和肌细胞生成素，以及生物钟蛋白。我们获得了在肌肉细胞系和骨骼肌中表达的蛋白质的绝对丰度，这应是一份有价值的资源。与组织相比，对葡萄糖摄取信号通路以及葡萄糖和脂质代谢途径中的蛋白质异构体进行定量，提供了该细胞系详细的代谢图谱。这揭示了在酶异构体水平上，肌肉组织中AMP激活的蛋白激酶和胰岛素信号传导存在意想不到的复杂调控。
