骨骼肌蛋白质组学：考量与机遇

Skeletal muscle proteomics: considerations and opportunities.

作者信息

Wong Julian P H, Ng Yaan-Kit, Kjærgaard Jeppe, Blazev Ronnie, Deshmukh Atul S, Parker Benjamin L

机构信息

Department of Anatomy and Physiology, The University of Melbourne, Melbourne, VIC, Australia.

Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.

出版信息

NPJ Metab Health Dis. 2025 Jul 2;3(1):30. doi: 10.1038/s44324-025-00073-2.

DOI:10.1038/s44324-025-00073-2

PMID:40604168

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12222842/

Abstract

Skeletal muscle accounts for 30-40% of body weight and plays an indispensable role in maintaining movement and is also a central regulator of whole-body metabolism. As such, understanding the molecular mechanisms of skeletal muscle health and disease is vital. Proteomics has been revolutionized in recent years and provided new insights into skeletal muscle. In this review, we first highlight important considerations unique to the field which make skeletal muscle one of the most challenging tissues to analyse by mass spectrometry. We then highlight recent advances using the latest case studies and how this has allowed coverage of the skeletal muscle temporal, fibre type and stem cells proteome. We also discuss how exercise and metabolic dysfunction can remodel the muscle proteome. Finally, we discuss the future directions of the field and how they can be best leveraged to increase understanding of human biology.

摘要

骨骼肌占体重的30 - 40%，在维持运动中发挥着不可或缺的作用，也是全身代谢的核心调节因子。因此，了解骨骼肌健康与疾病的分子机制至关重要。近年来蛋白质组学发生了变革，为骨骼肌研究提供了新的见解。在本综述中，我们首先强调该领域特有的重要考量因素，这些因素使得骨骼肌成为通过质谱分析最具挑战性的组织之一。然后，我们通过最新的案例研究突出近期进展，以及这如何实现对骨骼肌时间、纤维类型和干细胞蛋白质组的覆盖。我们还讨论了运动和代谢功能障碍如何重塑肌肉蛋白质组。最后，我们讨论该领域的未来方向，以及如何最好地利用这些方向来增进对人类生物学的理解。