Sugarcane Breeding Institute (ICAR), Coimbatore, Tamil Nadu, India.
Indian Grass and Fodder Research Institute Regional Station, Avikanagar, Rajasthan, India.
Plant Sci. 2015 Mar;232:23-34. doi: 10.1016/j.plantsci.2014.12.012. Epub 2014 Dec 23.
Heat shock proteins (HSPs) have a major role in stress tolerance mechanisms in plants. Our studies have shown that the expression of HSP70 is enhanced under water stress in Erianthus arundinaceus. In this paper, we evaluate the effects of overexpression of EaHSP70 driven by Port Ubi 2.3 promoter in sugarcane. The transgenic events exhibit significantly higher gene expression, cell membrane thermostability, relative water content, gas exchange parameters, chlorophyll content and photosynthetic efficiency. The overexpression of EaHSP70 transgenic sugarcane led to the upregulation of stress-related genes. The transformed sugarcane plants had better chlorophyll retention and higher germination ability than control plants under salinity stress. Our results suggest that EaHSP70 plays an important role in sugarcane acclimation to drought and salinity stresses and its potential for genetic engineering of sugarcane for drought and salt tolerance.
热休克蛋白（HSPs）在植物的应激耐受机制中起着重要作用。我们的研究表明，在斑茅中 HSP70 的表达在水分胁迫下增强。在本文中，我们评估了由 Port Ubi 2.3 启动子驱动的 EaHSP70 在甘蔗中的过表达的效果。转基因事件表现出明显更高的基因表达、细胞膜热稳定性、相对水分含量、气体交换参数、叶绿素含量和光合作用效率。EaHSP70 的过表达转基因甘蔗导致与应激相关的基因上调。在盐胁迫下，转化的甘蔗植物比对照植物具有更好的叶绿素保持能力和更高的发芽能力。我们的结果表明，EaHSP70 在甘蔗适应干旱和盐胁迫中起着重要作用，并且它在甘蔗抗旱和耐盐性的遗传工程中具有潜力。
