Shen Z W, Sun C R, Cao K M, Zhan S X
Yi Chuan Xue Bao. 1989;16(6):430-5.
The pure rice (Oryza sativa, subsp. Indica, cv. Zhenshan 97B) chloroplast DNA was digested by restriction enzyme BamHI and the resulting fragments were ligated to the BamHI site of pBR322. One recombinant plasmid which contains a 19.3kb insertel DNA fragment was isolated from the clone bank and was named pOSB1. A physical map of the recombinant plasmid was constructed by cleavage with ten restriction endonucleases, and the gene rbcL was located on the pOSB1.
用限制性内切酶BamHI消化纯水稻（Oryza sativa，亚种Indica，品种珍汕97B）叶绿体DNA，所得片段连接到pBR322的BamHI位点。从克隆文库中分离出一个含有19.3kb插入DNA片段的重组质粒，命名为pOSB1。用十种限制性内切核酸酶切割构建重组质粒的物理图谱，并将rbcL基因定位在pOSB1上。
