Moon E, Kao T H, Wu R
Nucleic Acids Res. 1987 Jan 26;15(2):611-30. doi: 10.1093/nar/15.2.611.
We describe the isolation of two rice chloroplast HindIII fragments (9.5 kb and 5.3 kb) each containing a gene cluster coding for the large subunit of ribulose-1,5-bisphosphate carboxylase (rbcL), beta and epsilon subunits of ATPase (atpB and atpE), tRNAmet (trnM) and tRNAval (trnV). All five genes contained in the 9.5 kb fragment are potentially functional, whereas in the 5.3 kb fragment, rbcL is truncated and atpB is frame-shift mutated. The copy number of the 9.5 kb fragment is 10 times that of the 5.3 kb fragment, indicating that the two fragments are probably located on different chloroplast genomes and represent two different (major and minor) genomic populations. Thus, the rice chloroplast genome appears to be heterogeneous, contrary to general belief. We also describe the isolation of a rice mitochondrial HindIII fragment (6.9 kb) which contains an almost complete transferred copy of this chloroplast gene cluster. In this transferred copy, the coding sequences of rbcL, atpE and trnM contain perfectly normal reading frames, whereas atpB has become grossly defective and trnV is truncated.
我们描述了两个水稻叶绿体HindIII片段（9.5 kb和5.3 kb）的分离情况，每个片段都包含一个基因簇，该基因簇编码1,5 - 二磷酸核酮糖羧化酶的大亚基（rbcL）、ATP合酶的β和ε亚基（atpB和atpE）、tRNAmet（trnM）和tRNAval（trnV）。9.5 kb片段中包含的所有五个基因都可能具有功能，而在5.3 kb片段中，rbcL被截断，atpB发生了移码突变。9.5 kb片段的拷贝数是5.3 kb片段的10倍，这表明这两个片段可能位于不同的叶绿体基因组上，代表了两个不同的（主要和次要）基因组群体。因此，与普遍看法相反，水稻叶绿体基因组似乎是异质的。我们还描述了一个水稻线粒体HindIII片段（6.9 kb）的分离情况，该片段包含这个叶绿体基因簇几乎完整的转移拷贝。在这个转移拷贝中，rbcL、atpE和trnM的编码序列包含完全正常的阅读框，而atpB已严重缺陷，trnV被截断。
