Lee Jung-Ho, Kim Seong-Gil
Department of Physical Therapy, School of Medical & Public Health, Kyungdong University, Gangwon-do, Republic of Korea.
Department of Rehabilitation Science, Daegu University, Gyeongbuk, Republic of Korea.
Ultrasound Med Biol. 2015 Mar;41(3):790-6. doi: 10.1016/j.ultrasmedbio.2014.10.015. Epub 2015 Jan 22.
The study described here investigated the effects of extracorporeal shock wave therapy (ESWT) on functional recovery and neurotrophin-3 expression in the spinal cord after sciatic nerve injury in rats. Forty-five 8-wk-old rats were used and randomly divided into three groups: An experimental group, a control group and a sham group. The experimental group received ESWT after the nerve-crushing damage. The sciatic functional index and Dartfish Software were used to determine the effect of sciatic nerve damage on functional changes. A 1-cm length of spinal cord encompassing the L4-6 level was removed for Western blot analysis. The sciatic functional index significantly changed in both the ESWT and control groups after impairment. In the time course evaluation of the ankle angle in the toe off, the ESWT group had statistically significant increases from day 21 onward. There was a significant difference in neurotrophin-3 expression between the groups on days 1, 7 and 14 after impairment. Early application of ESWT increased the expression of neurotrophin-3 and neurotrophin-3 mRNA, and daily therapy facilitated the activity of macrophages and Schwann cells, which affect the survival and regeneration of neurons.
本文所述研究探讨了体外冲击波疗法（ESWT）对大鼠坐骨神经损伤后脊髓功能恢复及神经营养因子-3表达的影响。选用45只8周龄大鼠，随机分为三组：实验组、对照组和假手术组。实验组在神经挤压损伤后接受ESWT治疗。采用坐骨神经功能指数和Dartfish软件来确定坐骨神经损伤对功能变化的影响。取包含L4-6节段的1厘米长脊髓用于蛋白质免疫印迹分析。损伤后，ESWT组和对照组的坐骨神经功能指数均发生显著变化。在离地时踝关节角度的时间进程评估中，ESWT组从第21天起有统计学意义的增加。损伤后第1、7和14天，各组之间神经营养因子-3表达存在显著差异。早期应用ESWT可增加神经营养因子-3及其mRNA的表达，每日治疗可促进巨噬细胞和雪旺细胞的活性，这会影响神经元的存活和再生。
